Amino acid facilitates absorption of copper in the Caco-2 cell culture model
Amino acid facilitates absorption of copper in the Caco-2 cell culture model
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DOI：
10.1016/j.lfs.2014.05.021
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发表时间：
2014-07-25
期刊：
影响因子：
6.1
通讯作者：
Hu, Ming
中科院分区：
文献类型：
--
作者：
Gao, Song;Yin, Taijun;Hu, Ming
目的：缺铜可导致致命的血液学和神经系统疾病或其他疾病。氨基酸参与铜离子的吸收。本研究的目的是在Caco-2细胞培养模型中评估氨基酸复合物形式对铜的吸收并确定其机制。主要方法：采用人结肠腺癌细胞Caco-2培养模型，测定无机形态（CuSO4）和氨基酸络合物形态铜离子的通透性。赖氨酸和蛋氨酸以及卡铂被用来确定氨基酸转运蛋白或铜转运蛋白1 （CTR1）的可能参与。主要发现：结果表明，所有的氨基添加复合物形式促进铜的吸收。铜离子在这些络合形态下的表观渗透率至少是CuSO4形态下的7.6倍。铜在各种氨基酸络合物形式中的渗透性排序为：谷氨酸铜<赖氨酸铜=天冬氨酸铜=蛋氨酸铜<精氨酸铜<赖氨酸/谷氨酸铜。机制研究表明，铜氨基酸络合物中铜的吸收增强可能是吸收增强（如铜-蛋氨酸络合物）或基底外侧外排增强（如铜-赖氨酸络合物）的结果。铜转运蛋白1（或CTR1）抑制剂卡铂不影响铜在cu -蛋氨酸络合物中的吸收，提示铜氨基酸络合物的主要途径不是CTR1。意义：氨基酸复合物中铜离子的吸收增强似乎是由氨基酸转运体介导的。(C) 2014爱思唯尔公司版权所有。
Aim: Copper deficiency could cause fatal hematological and neurological disorders or other diseases. Amino acids are involved in the absorption of copper ions. The purpose of this study is to evaluate the absorption of copper in amino acid complex forms and determine its mechanism in the Caco-2 cell culture model.Main methods: The human colonic adenocarcinoma cell line Caco-2 culture model was used to determine the permeability of copper ions in inorganic form (CuSO4) and the amino acid complex forms. Lysine and methionine, as well as carboplatin were used to determine the possible involvement of amino acid transporters or copper transporter 1 (CTR1).Key findings: The results showed that all of the amino add complex forms facilitated copper absorption. The apparent permeabilities of copper ions in these complex forms were at least 7.6 fold higher than those in the CuSO4 form. The permeability rank order of copper in various amino acid complex forms was Cu-glutamate < Cu-lysine = Cu-aspartic acid = Cu methionine < Cu-arginine < Cu-(lysine/glutamate). Mechanistic studies revealed that the enhanced absorption of copper in copper amino acid complexes could be the result of enhanced uptake (as in Cu-methionine complex) or enhanced basolateral efflux (as in Cu-lysine complex). Copper transporter 1 (or CTR1) inhibitor carboplatin did not affect the absorption of copper in Cu-methionine complex, suggesting that the dominant pathway for copper amino acid complexes is not CTR1.Significance: Enhanced absorption of copper ions in amino acid complex appears to be mediated by amino acid transporters. (C) 2014 Elsevier Inc. All rights reserved.