Dengue Virus-2 Infection Affects Fecundity and Elicits Specific Transcriptional Changes in the Ovaries of Aedes aegypti Mosquitoes.
Dengue Virus-2 Infection Affects Fecundity and Elicits Specific Transcriptional Changes in the Ovaries of Aedes aegypti Mosquitoes.
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DOI：
10.3389/fmicb.2022.886787
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发表时间：
2022
影响因子：
5.2
通讯作者：
Londoño-Renteria B
中科院分区：
文献类型：
--
作者：
Feitosa-Suntheimer F;Zhu Z;Mameli E;Dayama G;Gold AS;Broos-Caldwell A;Troupin A;Rippee-Brooks M;Corley RB;Lau NC;Colpitts TM;Londoño-Renteria B
登革热（DF）由登革病毒（DENV）引起，是世界上最严重的虫媒病毒疾病，估计每年有4亿人感染。埃及伊蚊是DENV的主要媒介，并传播其他几种人类病原体，包括寨卡病毒，黄热病和基孔肯雅病毒。以往的研究表明，蚊子的病原体感染可以改变生殖适合度，揭示了特定的媒介-病原体相互作用是媒介能力的关键决定因素。然而，只有少数研究检测了DENV感染对A.埃及人，显示多次血食会导致寿命和生育能力下降。为了提供DENV感染对产卵和繁殖力的影响的更全面的分析，我们评估了与模拟吸血蚊子（对照组）相比DENV-2吸血蚊子（感染组）的产卵时间。我们证实了在第一个促性腺激素周期的繁殖力显着下降。为了进一步研究这种表型和潜在的DENV-2感染依赖的基因表达变化，我们对A.感染DENV-2的埃及人与模拟感染的蚊子。该分析揭示了几个DENV-2调节基因;其中，我们确定了一组12个代谢基因，我们使用逆转录定量PCR（RT-qPCR）进行了验证。有趣的是，发现在DENV感染的蚊子卵巢中上调的两个基因在伊蚊细胞系中表现出对DENV-2的抗病毒作用。总而言之，这项研究为病毒-载体界面提供了有用的见解，突出了在第一个促性腺周期中DENV-2感染期间蚊子卵巢中基因表达变化的重要性，引发了可能干扰蚊子繁殖的抗病毒反应。 这一信息对于进一步研究A.埃及人对病毒的耐受能力，因为自然界中受病毒感染的蚊子是流行病期间支持病毒的强大来源，给公共卫生系统造成巨大负担。DENV-2感染改变了埃及伊蚊的第一个促性腺周期。
Dengue fever (DF), caused by the dengue virus (DENV), is the most burdensome arboviral disease in the world, with an estimated 400 million infections each year. The Aedes aegypti mosquito is the main vector of DENV and transmits several other human pathogens, including Zika, yellow fever, and chikungunya viruses. Previous studies have shown that the pathogen infection of mosquitoes can alter reproductive fitness, revealing specific vector-pathogen interactions that are key determinants of vector competence. However, only a handful of studies have examined the effect of DENV infection in A. aegypti, showing a reduction in lifespan and fecundity over multiple blood meals. To provide a more comprehensive analysis of the impact of DENV infection on egg laying and fecundity, we assessed egg laying timing in DENV-2 blood-fed mosquitoes (infected group) compared to mock blood-fed mosquitoes (control group). We confirmed a significant decrease in fecundity during the first gonadotrophic cycle. To further investigate this phenotype and the underlying DENV-2 infection-dependent changes in gene expression, we conducted a transcriptomic analysis for differentially expressed genes in the ovaries of A. aegypti infected with DENV-2 vs. mock-infected mosquitoes. This analysis reveals several DENV-2-regulated genes; among them, we identified a group of 12 metabolic genes that we validated using reverse transcription-quantitative PCR (RT-qPCR). Interestingly, two genes found to be upregulated in DENV-infected mosquito ovaries exhibited an antiviral role for DENV-2 in an Aedes cell line. Altogether, this study offers useful insights into the virus-vector interface, highlighting the importance of gene expression changes in the mosquito’s ovary during DENV-2 infection in the first gonadotrophic cycle, triggering antiviral responses that may possibly interfere with mosquito reproduction. This information is extremely relevant for further investigation of A. aegypti’s ability to tolerate viruses since virally infected mosquitoes in nature constitute a powerful source of supporting viruses during intra-epidemic periods, causing a huge burden on the public health system. Aedes aegypti first gonadotrophic cycle is altered by DENV-2 infection.
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DOI：
10.4269/ajtmh.16-0222
发表时间：
2016-10-05
期刊：
The American journal of tropical medicine and hygiene
影响因子：
--
作者：
Halstead SB
通讯作者：
Halstead SB
影响因子：
3.2
作者：
Araujo, Ricardo Vieira Y.;Feitosa-Suntheimer, Fabiana;Colpitts, Tonya M.
通讯作者：
Colpitts, Tonya M.
影响因子：
2.6
作者：
Campbell, Jacob B.;Overby, Paula F.;Harrison, Jon F.
通讯作者：
Harrison, Jon F.
影响因子：
9.8
作者：
Isoe, Jun;Koch, Lauren E.;Miesfeld, Roger L.
通讯作者：
Miesfeld, Roger L.
影响因子：
5.8
作者：
Ahmed, AM;Hurd, H
通讯作者：
Hurd, H