Stable isotope dilution assay (SIDA) and HS-SPME-GCMS quantification of key aroma volatiles for fruit and sap of Australian mango cultivars
Stable isotope dilution assay (SIDA) and HS-SPME-GCMS quantification of key aroma volatiles for fruit and sap of Australian mango cultivars
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DOI：
10.1016/j.foodchem.2016.11.130
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发表时间：
2017-04-15
期刊：
影响因子：
8.8
通讯作者：
Smyth, Heather E.
中科院分区：
文献类型：
--
作者：
San, Anh T.;Joyce, Daryl C.;Smyth, Heather E.
本文报道的是一种高通量的方法来量化在一个单一的分析的关键挥发物，有助于在澳大利亚种植的商业上重要的芒果品种的香气。该方法构成稳定同位素稀释分析（SIDA）结合顶空（HS）固相微萃取（SPME）耦合气相色谱质谱（GCMS）。氘标记的目标分析物的类似物是商业购买或合成用作内标。目标是7种挥发物，即己醛、3-<$烯、α-萜品烯、对伞花烃、柠檬烯、α-萜品油烯和辛酸乙酯。得到的校准函数的决定系数（R-2）范围为0.93775至0.99741。也实现了高回收率的加标芒果样品。应用该方法对“肯辛顿骄傲”和“B74”芒果果实以及“蜂蜜黄金”芒果汁液中的主要香气成分进行了鉴定。这种方法是一个显着的改进，目前的方法检测和测量芒果果实和树液挥发物的浓度。(C)2016爱思唯尔有限公司版权所有
Reported herein is a high throughput method to quantify in a single analysis the key volatiles that contribute to the aroma of commercially significant mango cultivars grown in Australia. The method constitutes stable isotope dilution analysis (SIDA) in conjunction with headspace (HS) solid-phase microextraction (SPME) coupled with gas-chromatography mass spectrometry (GCMS). Deuterium labelled analogues of the target analytes were either purchased commercially or synthesised for use as internal standards. Seven volatiles, hexanal, 3-carene, alpha-terpinene, p-cymene, limonene, a-terpinolene and ethyl octanoate, were targeted. The resulting calibration functions had determination coefficients (R-2) ranging from 0.93775 to 0.99741. High recovery efficiencies for spiked mango samples were also achieved. The method was applied to identify the key aroma volatile compounds produced by 'Kensington Pride' and 'B74' mango fruit and by 'Honey Gold' mango sap. This method represents a marked improvement over current methods for detecting and measuring concentrations of mango fruit and sap volatiles. (C) 2016 Elsevier Ltd. All rights reserved.