Intra-crystalline protein diagenesis (IcPD) in Patella vulgata. Part I: Isolation and testing of the closed system.
Intra-crystalline protein diagenesis (IcPD) in Patella vulgata. Part I: Isolation and testing of the closed system.
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DOI：
10.1016/j.quageo.2012.03.016
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发表时间：
2013-04
影响因子：
2.7
通讯作者：
Penkman KE
中科院分区：
文献类型：
--
作者：
Demarchi B;Rogers K;Fa DA;Finlayson CJ;Milner N;Penkman KE
本研究成功地分离出一部分的晶体内蛋白质的海洋腹足类髌骨vulgata的外壳，并评估这些蛋白质的ICPD（晶内蛋白质成岩作用）地质年代学的适用性。我们讨论的矿物组成的腹足类，首次通过X-射线衍射图调查，并使用结果来通知我们的采样策略。潜在的方解石边缘和散装样品（包含顶点和边缘）的壳作为稳定的储存库的晶内蛋白质在成岩作用进行检查。从壳内不同位置分离的晶内蛋白质的组成和成岩行为进行了比较，突出了针对一致的采样位置的必要性。我们通过在含水环境中进行高温实验，诱导了晶内和全壳蛋白质的人工成岩作用;这使我们能够量化氨基酸的沥滤损失，从而评估不同组分蛋白质的开放或封闭系统行为。所获得的结果提供了进一步的确认，成岩作用的模式根据蛋白质序列，结构和位置内或外的晶内馏分。由于髌骨是经常发现的化石记录，无论是在考古和地质背景下，ICPD地质年代学的应用，这种生物矿物开辟了可能性，以获得可靠的年龄信息，从一系列网站在世界不同地区。成功地从普通髌骨中分离出一种晶体内蛋白质组分。⑶通过X射线衍射研究P. vulgata的矿物组成。对普通小麦AAR年代学取样策略的优化。测试晶内蛋白质在成岩作用期间作为封闭系统。普通小麦是一种适合于ICPD年代学的基质。
This study successfully isolates a fraction of intra-crystalline proteins from shells of the marine gastropod Patella vulgata and assesses the suitability of these proteins for IcPD (Intra-crystalline Protein Diagenesis) geochronology. We discuss the mineralogical composition of this gastropod, investigated for the first time by X-ray diffraction mapping, and use the results to inform our sampling strategy. The potential of the calcitic rim and of a bulk sample (containing both apex and rim) of the shell to act as stable repositories for the intra-crystalline proteins during diagenesis is examined. The composition and the diagenetic behaviour of the intra-crystalline proteins isolated from different locations within the shell are compared, highlighting the necessity of targeting consistent sampling positions. We induced artificial diagenesis of both intra-crystalline and whole-shell proteins by conducting high-temperature experiments in hydrous environment; this allowed us to quantify the loss of amino acids by leaching and therefore evaluate the open- or closed-system behaviour of the different fractions of proteins. The results obtained provide further confirmation that patterns of diagenesis vary according to the protein sequence, structure, and location within or outside the intra-crystalline fraction. As Patella is frequently found in the fossil record, both in archaeological and geological contexts, the application of IcPD geochronology to this biomineral opens up the possibility to obtain reliable age information from a range of sites in different areas of the world. ► Successful isolation of an intra-crystalline fraction of proteins from Patella vulgata. ► Investigation of the mineralogical composition of P. vulgata by X-ray diffraction. ► Optimisation of the sampling strategy for AAR geochronology on P. vulgata. ► Testing of the intra-crystalline proteins as a closed system during diagenesis. ► P. vulgata is shown as a suitable substrate for IcPD geochronology.
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影响因子：
16.6
作者：
Coelfen, Helmut
通讯作者：
Coelfen, Helmut
DOI：
10.1016/s0022-0981(01)00211-8
发表时间：
2001-03-30
影响因子：
2
作者：
Jenkins, SR;Hartnoll, RG
通讯作者：
Hartnoll, RG
影响因子：
2.7
作者：
Crisp, Molly;Demarchi, Beatrice;Penkman, Kirsty
通讯作者：
Penkman, Kirsty
影响因子：
4
作者：
BOWEN, DQ;SYKES, GA;HARE, PE
通讯作者：
HARE, PE
影响因子：
4.8
作者：
EVANS, MR;WILLIAMS, GA
通讯作者：
WILLIAMS, GA