Bioactive glass fillers reduce bacterial penetration into marginal gaps for composite restorations.
Bioactive glass fillers reduce bacterial penetration into marginal gaps for composite restorations.
复制标题
DOI：
10.1016/j.dental.2015.10.007
复制
发表时间：
2016-01
期刊：
影响因子：
--
通讯作者：
Kruzic JJ
中科院分区：
文献类型：
--
作者：
Khvostenko D;Hilton TJ;Ferracane JL;Mitchell JC;Kruzic JJ
已知生物活性玻璃（BAG）具有抗菌和再矿化特性；然而，使用BAG作为树脂基复合修复体的填充剂来减缓复发性龋病的研究尚未开展。因此，本研究的目的是研究在循环机械载荷下，树脂复合材料中添加15 wt%的BAG对细菌生物膜渗透到体外模拟牙齿填充物边缘间隙的影响。将人磨牙加工成约3mm厚的牙本质盘，放置1.5-2 mm深的复合修复体。通过不使用牙胶，允许沿边缘的一半形成狭窄的15-20微米宽的牙本质复合间隙。使用了两种不同的72 wt%填充复合材料，一种是15 wt% BAG填料（15BAG）和平衡硅化锶玻璃，一种是OX-50和硅化锶玻璃不填充BAG （0BAG - control）。两组样品的表面都生长有变形链球菌生物膜，并在生物反应器中进行了两周的测试，同时受到周期性机械负荷的影响。在确认生物膜活力后，将每个标本固定在戊二醛中，革兰氏阳性染色，装在树脂中并横截面显示间隙轮廓。0BAG和15BAG的生物膜渗透深度被量化为间隙深度的分数。数据采用学生t检验进行比较。与0BAG相比，15BAG样品的细菌渗透到边缘间隙的平均深度明显更小（~61%），在0BAG中，所有样品都观察到100%的渗透，在某些情况下，生物膜渗透到修复物的下方。包含树脂牙科复合材料减少生物膜渗透到模拟牙齿修复体的边缘间隙。这表明含有BAG的复合材料可能具有减缓修复边缘继发性蛀牙的发展和传播的潜力。
Bioactive glass (BAG) is known to possess antimicrobial and remineralizing properties; however, the use of BAG as a filler for resin based composite restorations to slow recurrent caries has not been studied. Accordingly, the objective of this study was to investigate the effect of 15 wt% BAG additions to a resin composite on bacterial biofilms penetrating into marginal gaps of simulated tooth fillings in vitro during cyclic mechanical loading. Human molars were machined into approximately 3 mm thick disks of dentin and 1.5–2 mm deep composite restorations were placed. A narrow 15–20 micrometer wide dentin-composite gap was allowed to form along half of the margin by not applying dental adhesive to that region. Two different 72 wt% filled composites were used, one with 15 wt% BAG filler (15BAG) and the balance silanated strontium glass and one filled with OX-50 and silanated strontium glass without BAG (0BAG – control). Samples of both groups had Streptococcus mutans biofilms grown on the surface and were tested inside a bioreactor for two weeks while subjected to periods of cyclic mechanical loading. After post-test biofilm viability was confirmed, each specimen was fixed in glutaraldehyde, gram positive stained, mounted in resin and cross-sectioned to reveal the gap profile. Depth of biofilm penetration for 0BAG and 15BAG was quantified as the fraction of gap depth. The data were compared using a Student’s t-test. The average depth of bacterial penetration into the marginal gap for the 15BAG samples was significantly smaller (~61%) in comparison to 0BAG, where 100% penetration was observed for all samples with the biofilm penetrating underneath of the restoration in some cases. BAG containing resin dental composites reduce biofilm penetration into marginal gaps of simulated tooth restorations. This suggests BAG containing composites may have the potential to slow the development and propagation of secondary tooth decay at restoration margins.