Identification of two related markers for common acute lymphoblastic leukemia as heat shock proteins.
Identification of two related markers for common acute lymphoblastic leukemia as heat shock proteins.
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常见急性淋巴细胞白血病的两个相关标记物热休克蛋白的鉴定。
DOI：
10.1172/jci114413
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Tueche,HJ
中科院分区：
文献类型：
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作者：
Strahler,JR;Kuick,R;Eckerskorn,C;Lottspeich,F;Richardson,BC;Fox,DA;Stoolman,LM;Hanson,CA;Nichols,D;Tueche,HJ
通过直接分析白血病细胞的多肽成分，我们以前已经检测到几种多肽，它们的表达局限于急性淋巴细胞白血病（ALL）。在这项研究中，我们提供的证据表明，两个多肽命名为L2和L4的结构相关，并代表新的标记常见的急性淋巴细胞白血病。部分氨基酸序列分析没有发现L2和L4之间的差异。所获得的序列对应于先前克隆的命名为hsp 27的人类基因，该基因在热休克处理后在多种细胞中表达。32Pi掺入研究表明，L4是hsp27的非磷酸化形式，L2是hsp27的磷酸化形式。这两种形式是诱导热休克在白血病和非白血病淋巴细胞。因此，在急性白血病中，常见的ALL亚型的独特特征是组成性表达代表主要细胞磷蛋白的多肽。图片
By direct analysis of the polypeptide constituents of leukemic cells, we have previously detected several polypeptides that are restricted in their expression to acute lymphoblastic leukemia (ALL). In this study, we provide evidence that two polypeptides designated L2 and L4 are structurally related and represent novel markers for common ALL. Partial amino acid sequence analysis did not uncover differences between L2 and L4. The sequences obtained correspond to a previously cloned human gene designated hsp 27 that is expressed, following heat shock treatment, in a variety of cells. 32Pi incorporation studies indicate that L4 is an unphosphorylated form and L2 is a phosphorylated form of hsp27. The two forms were inducible by heat shock in leukemic and nonleukemic lymphoid cells. Thus, in acute leukemia, the common ALL subtype is uniquely characterized by the constitutive expression of a polypeptide that represents a major cellular phosphoprotein.Images