Abnormal Membrane Lipid‐Related Properties during Maturation of Reticulocytes from Severely Anaemic Rats as Measured by Partition in Two‐Polymer Aqueous Phases
Abnormal Membrane Lipid‐Related Properties during Maturation of Reticulocytes from Severely Anaemic Rats as Measured by Partition in Two‐Polymer Aqueous Phases
复制标题
通过在双聚合物水相中的分配测量严重贫血大鼠网织红细胞成熟过程中异常的膜脂质相关特性
DOI：
10.1111/j.1365-2141.1978.tb01110.x
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发表时间：
1978
影响因子：
6.5
通讯作者：
G. Ascher
中科院分区：
文献类型：
--
作者：
H. Walter;E. J. Krob;G. Ascher
当混合时，葡聚糖和聚乙二醇的水溶液产生不混溶的液体两相系统。这样的系统适合于通过分区分离细胞，并适合于追踪细胞膜表面性质随分化、成熟和年龄的细微变化。通过适当操纵聚合物浓度和离子组成和浓度，可以选择某些膜性质（例如电荷相关的，脂质相关的），这将决定细胞的分配系数（即细胞对顶相或底相的相对亲和力或对界面的吸附）。结合放射性同位素标记和细胞逆流分布技术，我们检查了来自严重贫血大鼠（通过苯肼注射）的网织红细胞在反映膜脂质相关特性的双聚合物相系统中的分配行为。发现这种应激网织红细胞在其膜脂质方面是异常的，其不产生在成熟期间用正常网织红细胞观察到的分配系数的变化。源自这种应激网织红细胞的成熟红细胞也具有异常的脂质相关膜特性。与我们早期的结果一起，很明显，严重贫血中产生的红细胞在其寿命期间具有并保留异常的电荷相关和脂质相关的膜表面特性，并且不经历与正常成熟和老化相关的膜表面变化，如通过在两种聚合物水相系统中的分配所识别的。
Aqueous solutions of dextran and of polyethylene glycol when mixed give rise to immiscible, liquid two‐phase systems. Such systems are suitable for separating cells by partition and for tracing subtle changes in the cells’membrane surface properties as a function of differentiation, maturation and age. By appropriate manipulation of polymer concentration and ionic composition and concentration one can select certain membrane properties (e.g. charge‐associated, lipid‐related) that will determine the cells’partition coefficient (i.e. the cells’relative affinity for the top or bottom phase or adsorption to the interface). Combining radioisotopic labelling and cell countercurrent distribution techniques we have examined the partition behaviour of reticulocytes from severely anaemic rats (made so through phenylhydrazine‐injection) in two‐polymer phase systems which reflect membrane lipid‐related properties. It was found that such stress reticulocytes are abnormal with respect to their membrane lipid which does not produce the changes in partition coefficients observed with normal reticulocytes during maturation. The mature erythrocytes derived from such stress reticulocytes also have abnormal lipid‐related membrane properties. Together with our earlier results it is clear that red blood cells produced in severe anaemia have and retain abnormal charge‐associated and lipid‐related membrane surface properties during their life‐span and do not undergo the membrane surface changes associated with normal maturation and ageing as discerned by partition in two‐polymer aqueous phase systems.