Molecular epidemiology and evolution of enterovirus 71 strains isolated from 1970 to 1998
Molecular epidemiology and evolution of enterovirus 71 strains isolated from 1970 to 1998
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DOI：
10.1128/jvi.73.12.9969-9975.1999
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发表时间：
1999-12-01
影响因子：
5.4
通讯作者：
Pallansch, MA
中科院分区：
文献类型：
--
作者：
Brown, BA;Oberste, MS;Pallansch, MA
肠道病毒71型（EV71）（肠道病毒属，小核糖核酸病毒科）是手足口病（HFMD）的常见病因，也可能导致严重的神经系统疾病，例如脑炎和脊髓灰质炎样瘫痪，为了检查EV 71的遗传多样性和进化速度，我们确定并分析了完整的VPI序列，（891个核苷酸），1970年至1998年在美国和其他五个国家分离的113个EV71菌株，核苷酸序列比较显示EV71有三种不同的基因型，命名为A、B和C。基因型内的遗传变异（12%或更少的核苷酸差异）小于基因型间的变异（16.5%至19.7%）。所有三种基因型的菌株在推导的氨基酸序列中彼此至少94%相同。1970年在加州分离的EV 71原型株BrCr-CA-70是A基因型的唯一成员。1972年至1988年期间在美国和澳大利亚分离的菌株、1994年哥伦比亚分离的菌株以及1997年马来西亚大规模HFMD爆发的菌株均为基因型B的成员。尽管基因型B的菌株继续在世界其他地区传播，但自1988年以来在美国没有分离出任何菌株。基因型C包含1985年或之后在美国、加拿大、澳大利亚和中华民国分离的毒株。B和C基因型内的年进化速率估计为每个核苷酸约1.35 × 10（-2）替换，与脊髓灰质炎病毒观察到的速率相似。结果表明EV 71是一种遗传多样性、快速进化的病毒，它在世界范围内的传播和引起严重疾病的潜力强调了需要额外的监测和改进的方法来识别人类疾病中的EV71。
Enterovirus 71 (EV71) (genus Enterovirus, family Picornaviridae), a common cause of hand, foot, and mouth disease (HFMD), may also cause severe neurological diseases, such as encephalitis and poliomyelitis-like paralysis, To examine the genetic diversity and rate of evolution of EV71, we have determined and analyzed complete VPI sequences (891 nucleotides) for 113 EV71 strains isolated in the United States and five other countries from 1970 to 1998, Nucleotide sequence comparisons demonstrated three distinct EV71 genotypes, designated A, B, and C, The genetic variation within genotypes (12% or fewer nucleotide differences) was less than the variation between genotypes (16.5 to 19.7%). Strains of all three genotypes were at least 94% identical to one another in deduced amino acid sequence. The EV71 prototype strain, BrCr-CA-70, isolated in California in 1970, is the sole member of genotype A. Strains isolated in the United States and Australia during the period from 1972 to 1988, a 1994 Colombian isolate, and isolates from a large HFMD outbreak in Malaysia in 1997 are all members of genotype B, Although strains of genotype B continue to circulate in other parts of the world, none have been isolated in the United States since 1988. Genotype C contains strains isolated in 1985 or later in the United States, Canada, Australia, and the Republic of China, The annual rate of evolution within both the B and C genotypes was estimated to be approximately 1.35 x 10(-2) substitutions per nucleotide and is similar to the rate observed for poliovirus, The results indicate that EV71 is a genetically diverse, rapidly evolving virus, Its worldwide circulation and potential to cause severe disease underscore the need for additional surveillance and improved methods to identify EV71 in human disease.