Anti-photoaging effects of chitosan oligosaccharide in ultraviolet-irradiated hairless mouse skin
Anti-photoaging effects of chitosan oligosaccharide in ultraviolet-irradiated hairless mouse skin
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DOI：
10.1016/j.exger.2017.12.018
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发表时间：
2018-03-01
影响因子：
3.9
通讯作者：
Li, Si-Dong
中科院分区：
文献类型：
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作者：
Kong, Song-Zhi;Li, Dong-Dong;Li, Si-Dong
皮肤光老化（SP）是反复暴露于紫外线（UV）辐射后出现的一种过早的皮肤老化损伤，主要特征是氧化应激和炎症失平衡，使皮肤呈现粗皱纹、干燥、色素不规则、松弛等光老化的典型症状。壳寡糖(COS)是一种具有良好保湿性能的天然多糖，是壳聚糖的解聚产物，具有多种生物活性，其中抗氧化、抗炎作用近年来被频繁报道。然而，现有的体内研究还没有表明COS是否对紫外线诱导的SP有直接的保护作用。在当前的研究中，我们研究了 COS 对无毛小鼠背部皮肤紫外线造成的损伤的潜在预防作用。数据显示，每次紫外线照射后局部涂抹COS，持续10周，可有效抑制紫外线引起的皮肤不良变化。具体而言，COS通过减轻胶原纤维的破坏，提高I型胶原的相对含量和总胶原量，明显减轻小鼠皮肤的宏观和组织病理学损伤。此外，COS还能有效抑制TNF-α、IL-1β、IL-6等促炎细胞因子的水平，并显着提高抗氧化酶（SOD、GSH-Px、CAT）的活性以及皮肤羟脯氨酸和水分的含量。这些发现表明，这种天然多糖至少部分地通过抗氧化和抗炎状态的有利调节来减弱紫外线诱导的 SP，这可能协同作用以维持真皮胶原蛋白的形态和水平。
Skin photoaging (SP) is a premature skin-aging damage after repeated exposure to ultraviolet (UV) radiation, mainly characterized by oxidative stress and inflammatory disequilibrium, which makes skin show the typical symptoms of photoaging such as coarse wrinkling, dryness, irregular pigmentation and laxity. Chitosan oligosaccharide (COS), a natural polysaccharide with good humectant property, is the depolymerized product of chitosan with various biological activities, among which the antioxidant and anti-inflammatory effects have been frequently reported in recent years. However, no existing invivo study indicates whether COS has direct protective effect on UV-induced SP. In the current research, we investigated the potential preventive effect of COS against UV-caused damage in hairless mouse dorsal skin. The data showed that COS, by topical application after each UV-radiation for 10 weeks, effectively inhibited the undesirable changes on the skin induced by UV. To be specific, COS obviously alleviated the macroscopic and histopathological damages of mice skin, via mitigating the disrupted collagenous fibers, as well as improving the relative content of type I collagen and the amount of total collagen. Furthermore, COS effectively inhibited the levels of pro-inflammatory cytokines such as TNF-alpha, IL-1 beta and IL-6, and markedly improved the activities of antioxidant enzymes (SOD, GSH-Px, CAT), as well as the content of skin hydroxyproline and moisture. These findings demonstrated that this natural polysaccharide attenuated UV-induced SP, at least in part, by virtue of favorable regulation of antioxidant and anti-inflammatory status, which presumably worked in concert to maintain the morphology and level of dermal collagen.