The identification of outer membrane proteins and flagella of Campylobacter jejuni.
The identification of outer membrane proteins and flagella of Campylobacter jejuni.
复制标题
空肠弯曲杆菌外膜蛋白和鞭毛的鉴定。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
A. Pearson
中科院分区：
文献类型：
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作者：
D. Newell;H. McBride;A. Pearson
采用125 - i表面标记和SDS-PAGE外膜制剂对5株空肠弯曲杆菌临床分离株的外膜蛋白进行了鉴定。所有分离株均表达一种分子量不同的外膜蛋白（43 000-46 000:43 k -46 k）。几个恒定的表面蛋白也被鉴定出来，包括一个27K蛋白，它是表面暴露的，可以酸提取，但在外膜制备中不存在。鞭毛由一个主要的62K蛋白和一个次要的87K蛋白组成。这两种蛋白在鞭毛虫变异中都不存在。62K蛋白经兔抗鞭毛抗血清免疫印迹和免疫沉淀。
The outer membrane proteins of five clinical isolates of Campylobacter jejuni were identified by 125I-surface labelling and SDS-PAGE of outer membrane preparations. All isolates expressed a major outer membrane protein of variable molecular weight (43 000-46 000: 43K-46K). Several constant surface proteins were also identified including a 27K protein which was surface-exposed and acid-extractable but was not present in the outer membrane preparations. Isolated flagella comprised a major 62K protein and a minor 87K protein. Both proteins were absent in an aflagellate variant. The 62K protein was immunoblotted and immunoprecipitated by rabbit anti-flagella antisera.