MICROINJECTION OF FLUORESCENT BRAIN TUBULIN REVEALS DYNAMIC PROPERTIES OF CORTICAL MICROTUBULES IN LIVING PLANT-CELLS
MICROINJECTION OF FLUORESCENT BRAIN TUBULIN REVEALS DYNAMIC PROPERTIES OF CORTICAL MICROTUBULES IN LIVING PLANT-CELLS
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DOI：
10.1002/cm.970240308
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发表时间：
1993-01-01
影响因子：
--
通讯作者：
HEPLER, PK
中科院分区：
文献类型：
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作者：
WASTENEYS, GO;GUNNING, BES;HEPLER, PK
荧光脑微管蛋白被注射到绿藻Nitella pseudoflabellata和高等植物Tradescantia virginia的活细胞中，与皮层微管结合，使这些结构得以观察到。使用激光共聚焦扫描显微镜，记录了微管的清晰图像，并记录了微管模式的变化。注射后，微管的荧光长度在几分钟内出现，其数量和长度在前15分钟内迅速增加至“稳定状态”。在许多情况下，在注射后数小时仍可检测到荧光微管。在被检查的细胞中，微管被排列成一组单独的单元，只是偶尔与邻近的微管表现出密切的联系或精确的共排列。在我们所认为的稳态条件下，一些微管保持相对静止，而另一些则经历长度的快速变化或小的易位。我们还记录了在解聚后组装过程中出现的双向微管延伸。
Fluorescent brain tubulin, injected into living cells of the green alga Nitella pseudoflabellata and the higher plant Tradescantia virginiana, incorporates into the cortical microtubules, allowing these structures to be observed. With confocal laser scanning microscopy, clear images of microtubules were recorded and changes in microtubule patterns documented. After injection, fluorescent lengths of microtubules appeared within a few minutes and their number and length increased rapidly to a ''steady state'' over the first 15 min. In many instances, fluorescent microtubules could still be detected several hours after injection. In the cells examined, microtubules are arranged as an array of separate units only occasionally displaying close association or accurate co-alignment with neighboring microtubules. In what we perceive to be the steady state condition, some microtubules remain relatively static, while others undergo rapid changes in length or small translocations. We also document what appears to be bidirectional microtubule elongation during postdepolymerization assembly.