Alcohol Modulates the Biogenesis and Composition of Microglia-Derived Exosomes
Alcohol Modulates the Biogenesis and Composition of Microglia-Derived Exosomes
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DOI：
10.3390/biology8020025
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发表时间：
2019-06-01
期刊：
影响因子：
4.2
通讯作者：
Matthews, Qiana L.
中科院分区：
文献类型：
--
作者：
Crenshaw, Brennetta J.;Kumar, Sanjay;Matthews, Qiana L.
外泌体是一种小的细胞外囊泡，已成为细胞间通讯的重要工具。在中枢神经系统中，外泌体可以介导神经胶质和神经元的通讯。一旦从供体细胞释放，外泌体可以作为离散的囊泡并行进到远端和近端受体细胞以改变细胞功能。小胶质细胞由于酒精滥用的应激刺激而分泌外泌体。本研究的目的是研究酒精暴露对来自小胶质细胞系BV-2的外泌体的生物发生和组成的影响。将BV-2细胞在无外泌体的培养基中培养，并进行模拟处理（对照）或用50 mM或100 mM乙醇处理48和72 h。我们的研究结果表明，酒精显着影响BV-2细胞的形态，活力和蛋白质含量。最重要的是，我们的研究表明，外泌体的生物发生和组成受到酒精处理的影响。
Exosomes are small extracellular vesicles that have emerged as an important tool for intercellular communication. In the central nervous system, exosomes can mediate glia and neuronal communication. Once released from the donor cell, exosomes can act as discrete vesicles and travel to distant and proximal recipient cells to alter cellular function. Microglia cells secrete exosomes due to stress stimuli of alcohol abuse. The goal of this study was to investigate the effects of alcohol exposure on the biogenesis and composition of exosomes derived from microglia cell line BV-2. The BV-2 cells were cultured in exosome-free media and were either mock treated (control) or treated with 50 mM or 100 mM of alcohol for 48 and 72 h. Our results demonstrated that alcohol significantly impacted BV-2 cell morphology, viability, and protein content. Most importantly, our studies revealed that exosome biogenesis and composition was affected by alcohol treatment.