Effect of pentoxifylline on the intrinsic swimming forces of human sperm assessed by optical tweezers.
Effect of pentoxifylline on the intrinsic swimming forces of human sperm assessed by optical tweezers.
复制标题
DOI：
10.1002/j.1939-4640.2000.tb02144.x
复制
发表时间：
2000-09
影响因子：
--
通讯作者：
Pasquale Patrizio;Yagang Liu;G. Sonek;M. W. Berns;Y. Tadir
中科院分区：
文献类型：
--
作者：
Pasquale Patrizio;Yagang Liu;G. Sonek;M. W. Berns;Y. Tadir
在体外，将精子暴露于己酮可可碱是否会增加精子的活力和作用力，这一点被定义为速度与尾巴拍频的乘积，目前仍存在争议。在过去，激光光钳已被成功地用于评估基础条件下的精子力。这项前瞻性研究的目的是确定人类精子暴露于己酮可可碱是否会对精子内在力产生任何影响。12名接受常规精液分析的健康受试者参加了这项研究。10例精液参数正常，2例弱精子症。每个精液标本经过洗涤后，再悬浮在人输卵管液(HTF)中，分成2等份。其中一个等价物与己酮可可碱孵育30分钟(最终浓度=3.6 mM)；另一个等价物不含己酮可可碱，作为对照。30分钟后，将己酮可可碱处理后的等量分成2份，其中一份洗涤以去除己酮可可碱，另一份与化学物质长期共同孵育。主要结果是测量精子内力(以毫瓦为单位)，通过倒置显微镜中的1064 nm连续波激光产生的非侵入性红外激光光学陷阱进行评估。将精子暴露于己酮可可碱可持续增加精子从激光光学陷阱中逃逸的相对力量。与对照组相比，基线力增加了33%到154%。精子力量的平均绝对值从37 mW上升到79 mW(P<；0.05)。精子初始相对逃逸能力较低的样本获得了最高的相对增加。己酮可可碱对精子力量的影响在5分钟后就已经很明显了，在30分钟时达到峰值，并在共孵化过程中剩下的精子和洗去己酮可可碱的精子中持续了长达3个小时。总而言之，己酮可可碱显著增加正常精子和弱精子样本的精子内在力。这项实验证实了光钳可以在体外条件下准确地测定精子的力量。临床数据现在必须确定有记录的精子力增加是否是评估精子受精能力的重要参数。
It is still controversial whether in vitro exposure of sperm to pentoxifylline increases sperm motility and force, which is defined as the product of velocity by beat frequency of the tail. Laser optical tweezers have been successfully used in the past to evaluate sperm force in basal conditions. The aim of this prospective study was to determine whether exposure of human sperm to pentoxifylline has any effect on sperm intrinsic forces. Twelve healthy subjects undergoing routine semen analysis were enrolled in the study. Ten exhibited normal semen parameters, 2 exhibited asthenozoospermia. Each semen specimen was washed and, after swim-up, resuspended in human tubal fluid (HTF) and divided into 2 aliquots. One aliquot was incubated with pentoxifylline for 30 minutes (final concentration = 3.6 mM); the second aliquot, without pentoxifylline, served as a control. After 30 minutes the pentoxifylline-treated aliquot was divided into 2 portions, 1 of which was washed to remove the pentoxifylline, the other was left in prolonged coincubation with the chemical. The main outcome was the measurement of sperm intrinsic force in milliwatts (mW), which was assessed by means of a noninvasive infrared laser optical trap created by a continuous wave, 1064-nm Nd:YAG laser beam directed in an inverted microscope. Exposure of sperm to pentoxifylline consistently increased sperm relative escape force from the laser optical trap. The increase ranged from 33% to 154% over baseline force compared with controls. The average absolute increase in sperm force rose from 37 mW to 79 mW (P < .05). Specimens with sperm having an initial low relative escape force gained the highest relative increase. The effect of pentoxifylline on sperm force, already apparent after 5 minutes, reached a peak at 30 minutes and persisted for up to 3 hours in sperm that were left in coincubation and in those on which the pentoxifylline had been washed off. In conclusion, pentoxifylline significantly increases sperm intrinsic relative force in normozoospermic and asthenozoospermic samples. This experiment confirms that optical tweezers can provide an accurate determination of sperm force in in vitro conditions. Clinical data must now establish whether a documented increase in sperm force is an important parameter for assessing sperm fertilizing capacity.