Synthesis and properties of novel 2'-O-alkoxymethyl-modified nucleic acids
Synthesis and properties of novel 2'-O-alkoxymethyl-modified nucleic acids
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新型2-O-烷氧基甲基修饰核酸的合成及性质
DOI：
10.1016/j.bmcl.2011.09.003
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Arai;K.;Uchiyama;N.;Wada;T.
合成了具有烷氧基甲基骨架的新型2′-O-修饰寡核苷酸，并分析了它们与互补核酸杂交的能力和它们的核酸酶抗性。通过引入吸电子基团提高了杂化能力，并且对于氯取代的化合物，熔融温度（Tm值）的增加特别高。这些2′-O-烷氧基甲基化寡聚体的核酸酶抗性低于预期，但氰基取代导致比2′-O-甲基化更高的核酸酶抗性。
Novel 2′-O-modified oligoribonucleotides with alkoxymethyl skeletons were synthesized, and their ability to hybridize complementary nucleic acids and their nuclease resistance were analyzed. The hybridization ability was improved by introducing electron-withdrawing groups and the increases in melting temperature (Tmvalue) was particularly high for chlorine-substituted compounds. Nuclease resistance of these 2′-O-alkoxymethylated oligomers was lower than expected, but cyano substitution resulted in a higher nuclease resistance than 2′-O-methylation.