Screening of Indonesian medicinal plant extracts for antibabesial activity and isolation of new quassinoids from Brucea javanica.
Screening of Indonesian medicinal plant extracts for antibabesial activity and isolation of new quassinoids from Brucea javanica.
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DOI：
10.1021/np070236h
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发表时间：
2007-09
影响因子：
5.1
通讯作者：
Subeki;H. Matsuura;Kosaku Takahashi;K. Nabeta;M. Yamasaki;Y. Maede;K. Katakura
中科院分区：
文献类型：
--
作者：
Subeki;H. Matsuura;Kosaku Takahashi;K. Nabeta;M. Yamasaki;Y. Maede;K. Katakura
从28种印度尼西亚药用植物中提取的煮沸提取物进行了筛选，以确定其对吉氏巴贝斯虫的体外抗巴贝斯虫活性。在这些提取物中，在10 μ g/mL的浓度下，鸦胆子的果实在抑制寄生虫生长方面最活跃。利用生物活性导向的方法分离纯化了黑木耳果实提取物。javanica中分离出两个新的苦木素类化合物，即马钱子碱醇B和马钱子碱J，并根据它们的光谱数据和通过化学转化为已知化合物来阐明这些化合物的结构。此外，还分离出已知的苦木素bruceines A-D、bruceantinol和yadanzietine A。还在体外检查了抗巴贝虫活性，结果表明，马钱子碱A和马钱子安替诺比醋酸亚胺氮烯（一种临床上用于抗B的药物（IC 50 = 103 ng/mL））更有效。gibsoni，IC 50值分别为4和12 ng/mL。
Boiled extracts derived from 28 Indonesian medicinal plants were screened for their antibabesial activity against Babesia gibsoni in vitro. Of these extracts, the fruit of Brucea javanica was the most active in inhibiting parasite growth at a concentration of 10 microg/mL. Bioassay-guided fractionation of the fruit extract of Br. javanica led to the isolation of two new quassinoids, bruceantinol B and bruceine J, and the structures of these compounds were elucidated on the basis of their spectroscopic data and by chemical transformation to known compounds. In addition, the known quassinoids bruceines A-D, bruceantinol, and yadanziolide A were isolated. Antibabesial activities were also examined in vitro, and bruceine A and bruceantinol were shown to be more potent than diminazene aceturate, a drug (IC50 = 103 ng/mL) used clinically against B. gibsoni, with IC50 values of 4 and 12 ng/mL, respectively.