Cell proliferation and migration on collagen substrata in vitro.
Cell proliferation and migration on collagen substrata in vitro.
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作者：
S. Schor
作为细胞外基质对细胞行为影响研究的一部分，提供了有关 (a) 胶原蛋白膜、(b) 天然胶原纤维 3 维凝胶表面和 (c) 3 维胶原凝胶基质内细胞增殖和迁移的定量数据。研究发现胶原蛋白环境的性质会影响某些细胞类型的增殖，但不会影响其他细胞类型的增殖。例如，HeLa 细胞在所有胶原蛋白基质上以大致相同的速率增殖并达到相同的饱和细胞密度，而与凝胶表面或胶原蛋白膜上的细胞相比，人类皮肤成纤维细胞在 3 维胶原蛋白凝胶基质内生长得更慢。天然胶原纤维的 3 维凝胶还可用于研究凝胶表面上的细胞迁移，以及细胞从凝胶表面迁移（或“渗透”）到 3 维胶原基质中。已使用两种方法来获得有关细胞浸润到胶原凝胶中的定量信息，一种方法涉及从凝胶表面选择性去除细胞，而另一种方法则依赖于直接显微镜检查。在迄今为止检查的细胞中，上皮细胞（正常和肿瘤）不表现出浸润行为，而正常和病毒转化的成纤维细胞以及非上皮来源的肿瘤细胞（例如黑色素瘤）确实浸润到胶原凝胶基质中，其渗透率根据细胞类型而有很大差异。
Quantitative data are presented regarding cell proliferation and migration on (a) collagen films (b) the surface of 3-dimensional gels of native collagen fibres and (c) within the 3-dimensional collagen gel matrix, as part of a study of the effects of the extracellular matrix on cell behaviour. The nature of the collagen environment was found to influence the proliferation of certain cell types, but not of others. For example, HeLa cells proliferate at approximately the same rate and reach the same saturation cell densities on all of the collagen substrata, while human skin fibroblasts grow more slowly within the 3-dimensional collagen gel matrix compared with cells either on the gel surface or on collagen films. The 3-dimensional gels of native collagen fibres may also be used to study cell migration on the gel surface, as well as cell migration (or 'infiltration') from the gel surface into the 3-dimensional collagen matrix. Two methods have been used to obtain quantitative information concerning cell infiltration into the collagen gel, one involving the selective removal of cells from the gel surface, while the other relies on direct microscopic examination. Of the cells examined to date, epithelial cells (both normal and tumour) do not show infiltrative behaviour, while both normal and virally transformed fibroblasts, as well as tumour cells of non-epithelial origin (e.g. melanoma), do infiltrate into the collagen gel matrix, at rates which vary considerably according to cell type.