Mapping the mechanical pulse of single cardiomyocytes with the atomic force microscope
Mapping the mechanical pulse of single cardiomyocytes with the atomic force microscope
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DOI：
10.1007/s002490050198
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发表时间：
1999-01-01
影响因子：
2
通讯作者：
Radmacher, M
中科院分区：
文献类型：
--
作者：
Domke, J;Parak, WJ;Radmacher, M
用原子力显微镜（AFM）观察了鸡胚心肌细胞的收缩行为。通过观察活性单细胞以及汇合层中的细胞来分析心肌细胞的机械脉动。当嵌入融合层中时，由于同步，细胞的脉冲通常被发现在节拍的频率和振幅方面非常稳定，包括负振幅和正振幅。然而，由于层内收缩中心的运动，振幅符号的翻转有时也会在分钟的时间尺度上发生。相比之下，单细胞经常在脉冲活跃期和完全安静期之间变化。还观察到特征参数如搏动周期和脉冲幅度不稳定。最后，我们结合了AFM对粘附的单细胞成像和记录局部搏动幅度的能力，以横向分辨单细胞的脉冲行为。
The atomic force microscope (AFM) was used to analyse the contractile behaviour of embryonic chicken cardiomyocytes. The mechanical pulsing of cardiomyocytes was analysed by observing active single cells as well as cells in a confluent layer. When embedded in a confluent layer, owing to synchronisation, pulsing of the cells was often found to be very stable in terms of frequency and amplitude of the beat, including negative as well as positive amplitudes. Nevertheless, owing to movements of contraction centres within the layer, a flipping of the sign of the amplitude did sometimes also occur on a time scale of minutes. In contrast, single cells often changed between active periods of pulsing and periods of complete quietness. Also characteristic parameters like beat period and pulse amplitude were observed to be unstable. Finally, we combined the abilities of the AFM to image adherent single cells and to record locally beat amplitudes, to characterise the pulsing behaviour of single cells laterally resolved.