The secondary structure of the 5'-noncoding region of beet necrotic yellow vein virus RNA 3: evidence for a role in viral RNA replication.
The secondary structure of the 5'-noncoding region of beet necrotic yellow vein virus RNA 3: evidence for a role in viral RNA replication.
复制标题
甜菜坏死黄脉病毒 RNA 3 5-非编码区的二级结构：在病毒 RNA 复制中作用的证据。
DOI：
10.1093/nar/21.6.1389
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发表时间：
1993
影响因子：
14.9
通讯作者：
B. Ehresmann
中科院分区：
文献类型：
--
作者：
David Gilmer;Christine Allmang;Chantal Ehresmann;H. Guilley;Kenneth Richards;G. Jonard;B. Ehresmann
利用二级结构敏感的化学和酶探针建立了甜菜坏死黄静脉病毒RNA 3长5'非编码区前312个残基的折叠模型。该结构由两个主要结构域组成，其中一个包括位于237和292位之间的两个短序列元件（框I和框II）和靠近5‘端的互补元件（框I’和框II'）之间的长距离碱基配对相互作用。先前的研究表明，这些序列元件（无论是正链RNA还是负链RNA）之间的碱基配对对于感染期间RNA 3的积累很重要。产生的RNA 3转录本含有突变，这些突变优先破坏了正链或负链上的Box II-II'碱基配对。在感染实验中，在正链结构中破坏Box II-II相互作用的突变转录本的复制效率低于在负链中破坏Box II-II相互作用的突变转录本。这些发现表明，Box II-II’相互作用促成的复合物5’-近端正链结构至少包含部分正链RNA合成的启动子。
Secondary structure-sensitive chemical and enzymatic probes have been used to produce a model for the folding of the first 312 residues of the long 5'-noncoding region of beet necrotic yellow vein virus RNA 3. The structure consists of two major domains, one of which includes long distance base-pairing interactions between two short sequence elements (Box I and Box II) situated between positions 237 and 292 and complementary elements (Box I' and II') near the 5'-terminus. Previous studies have shown that base pairing between these sequence elements (in either the plus-strand or minus-strand RNA) is important for RNA 3 accumulation during infection. RNA 3 transcripts were produced containing mutations which preferentially disrupted Box II-II' base pairing in either the plus- or minus-strand. In infection experiments, transcripts with mutations which disrupted the Box II-II' interaction in the plus-strand structure replicated less efficiently than mutants in which the Box II-II' interaction was disrupted in the minus-strand. These findings indicate that the complex 5'-proximal plus-strand structure to which the Box II-II' interaction contributes comprises at least part of the promoter for plus-strand RNA synthesis.