RCAS-RNAi: a loss-of-function method for the developing chick retina.
RCAS-RNAi: a loss-of-function method for the developing chick retina.
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RCAS-RNAI：一种发育中的鸡视网膜的功能丧失方法。
DOI：
10.1186/1471-213x-6-2
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发表时间：
2006-01-22
影响因子：
--
通讯作者：
Cepko, CL
中科院分区：
文献类型：
--
作者：
Harpavat, S;Cepko, CL
雏鸡为发育研究提供了一个极好的模型系统。然而，它缺乏一种有效的功能丧失方法来研究基因功能。我们证明禽逆转录病毒可以向发育中的鸡眼传递介导 RNA 干扰的发夹。这些病毒“敲低”视网膜感染区域的特定基因。随着视网膜的成熟，这种敲低现象持续存在，并且可以使用原位杂交来检测。此外，可以通过控制感染程度来控制受影响的视网膜组织的数量。该技术为发育中的小鸡视网膜的研究提供了快速有效的功能丧失选择。
The embryonic chick provides an excellent model system for studies of development. However, it has lacked an efficient loss-of-function method for studies of gene function. We show that avian retroviruses can deliver hairpins mediating RNA interference to the developing chick eye. These viruses 'knock down' specific genes in infected areas of the retina. The knock down persists as the retina matures and can be detected using in situ hybridization. Furthermore, the amount of retinal tissue affected can be controlled by manipulating the degree of infection. This technique provides a rapid and efficient loss-of-function option for studies in the developing chick retina.