Changes in the amount of cytoplasmic inclusions in mouse oocytes during meiotic maturation in vivo and in vitro.
Changes in the amount of cytoplasmic inclusions in mouse oocytes during meiotic maturation in vivo and in vitro.
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DOI：
10.1111/j.1447-0578.2004.00075.x
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发表时间：
2004-12-01
影响因子：
3.4
通讯作者：
Takano, Hiroko
中科院分区：
文献类型：
--
作者：
Niimura, Sueo;Kawakami, Shin-Ya;Takano, Hiroko
背景和目的：目前只在猪卵母细胞中研究了减数分裂成熟过程中细胞质包涵体的变化。本研究检测了小鼠卵母细胞在体内和体外成熟过程中胞质包涵体（糖原颗粒、脂滴和纤维结构）的数量和数量。对于那些在成熟过程中数量发生变化的包涵体，我们也检测了它们在经olomoucine（一种细胞周期蛋白依赖性激酶抑制剂）处理的卵母细胞中的含量，以阐明核成熟与包涵体变化之间的关系。方法：对不同时期培养的卵母细胞和取自窦卵泡和输卵管的卵母细胞进行核成熟染色。为了显示糖原颗粒和脂滴，卵母细胞采用周期性酸-希夫染色或苏丹染色。电镜下观察卵母细胞的纤维结构。结果：在体内和体外成熟的卵母细胞中，糖原颗粒的数量、亲苏丹性脂滴的数量和纤维结构没有变化，而脂滴的数量随着成熟而增加。在奥洛穆辛处理的卵母细胞中，核成熟的恢复受到抑制，而亲苏丹性脂滴数量的增加不受抑制。结论：本研究结果提示，无论在体内还是体外成熟，小鼠卵母细胞成熟过程中细胞质中亲苏丹性脂滴数量的增加都发生在细胞质中，并且这种包涵的变化与核成熟无关。（生殖医学，2004;3:231-236）。
Background and aims: The changes in cytoplasmic inclusions during meiotic maturation have only been examined in porcine oocytes. In the present study, the amount and the number of cytoplasmic inclusions (glycogen granules, lipid droplets and fibrous structures) were examined in mouse oocytes in the process of in vivo and in vitro maturation. For those inclusions that changed in amount during maturation, we also examined their content in oocytes treated with olomoucine, an inhibitor of cyclin-dependent kinase, in order to clarify the relationship between nuclear maturation and changes in the inclusions. Methods: Nuclear maturation in the oocytes cultured for various periods and those collected from antral follicles and oviducts was examined after staining with aceto-orcein. For the demonstration of glycogen granules and lipid droplets, oocytes were stained with periodic acid-Schiff or Sudan IV. Fibrous structures in the oocytes were observed under an electron microscope. Results: The amount of glycogen granules, Sudanophilic lipid droplets and fibrous structures did not change in the oocytes matured in vivo and in vitro, whereas the number of the lipid droplets increased during maturation. In the oocytes treated with olomoucine, the resumption of nuclear maturation was inhibited, whereas the increase in the number of Sudanophilic lipid droplets was not inhibited. Conclusion: Present findings suggest that the increase in the number of Sudanophilic lipid droplets occurs in the cytoplasm of mouse oocytes during maturation, regardless of in vivo or in vitro maturation, and that such the change in the inclusion is not related to nuclear maturation. (Reprod Med Biol 2004; 3: 231-236).