Protective effects of Forsythia suspensa extract against oxidative stress induced by diquat in rats
Protective effects of Forsythia suspensa extract against oxidative stress induced by diquat in rats
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连翘提取物对敌草快诱导的大鼠氧化应激的保护作用
DOI：
10.1016/j.fct.2009.12.018
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发表时间：
2010-02-01
影响因子：
4.3
通讯作者：
Kim, S. W.
中科院分区：
文献类型：
--
作者：
Lu, T.;Piao, X. L.;Kim, S. W.
连翘提取物是一种潜在的抗氧化剂。本研究旨在获得最佳的体外抗氧化组分，并在雄性Sprague道利大鼠体内检测其抗氧化潜力。1，1-二苯基-2-苦肼基（DPPH）自由基清除实验表明，F suspensa（FSC）的CH_2Cl_2组分清除DPPH自由基的活性最强，其中的连翘酯苷A、连翘酯苷A和连翘苷元可能是FSC的主要抗氧化成分。体内;与阴性对照组相比，用不同剂量的FSC（25、50和100 mg/kg bw）和维生素C（100 mg/kg bw，阳性对照）预处理大鼠15天显著降低了血浆中的肿瘤坏死因子-α（TNF-α）、白细胞介素-1 β（IL-1 β）和白细胞介素-6（IL-6）。FSC还能显著提高血浆、肝、肾组织中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性和谷胱甘肽（GSH）含量，降低血浆和肾组织中丙二醛（MDA）含量。FSC（100 mg/kg bw）的保护效果优于维生素C。这些结果表明，FSC对敌百虫诱导的氧化应激具有保护作用，值得成为一种潜在的膳食抗氧化剂。(C)2009爱思唯尔有限公司版权所有
Forsythia suspensa extract has been proved as a potential antioxidant in the recent years. The present study was undertaken to obtain the optimal antioxidant fraction in vitro and examine its antioxidative potential against diquat-induced oxidative stress in male Sprague Dawley rats in vivo. In vitro, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging experiment indicated that the CH2Cl2 fraction of F suspensa (FSC) exerted the strongest scavenging activities; forsythoside A, forythialan A and phillygenin from it might be the major antioxiclant constituents. In vivo; pretreatment of rats with different doses of FSC (25, 50 and 100 mg/kg bw) and vitamin C (100 mg/kg bw, positive control) for 15 days significantly lowered the tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and interleukin-6 (IL-6) in plasma compared to the negative control group. Also, FSC significantly increased the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and the levels of glutathione (GSH) in plasma, liver and kidney whereas it decreased the levels of malondialdehyde (MDA) in plasma and kidney. Moreover, the protective effect of FSC (100 mg/kg bw) was better than vitamin C. These results revealed that FSC exerted a protective effect against diquat-induced oxidative stress and is worthy of becoming a potential dietary antioxidant. (C) 2009 Elsevier Ltd. All rights reserved