Anatomical basis of sun compass navigation I: The general layout of the monarch butterfly brain
Anatomical basis of sun compass navigation I: The general layout of the monarch butterfly brain
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DOI：
10.1002/cne.23054
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发表时间：
2012-06-01
影响因子：
2.5
通讯作者：
Reppert, Steven M.
中科院分区：
文献类型：
--
作者：
Heinze, Stanley;Reppert, Steven M.
每年秋天，北美东部的帝王蝶（Danaus plexippus）利用时间补偿太阳罗盘迁移到墨西哥中部的越冬地。太阳罗盘机制涉及到神经整合的天窗线索与定时信息从昼夜节律钟，以保持一个恒定的航向。中枢复合体（中枢脑的一个重要结构）中的罗盘神经元与生物钟之间必要的相互作用的神经元基质在很大程度上是未知的。为了开始解开这些神经基质，我们进行了3D重建的所有神经柱的君主大脑的基础上抗突触蛋白标记。我们的工作特点21个定义明确的neuropils（19配对，2不配对），以及所有的突触区域之间的更经典的定义neuropils。我们还研究了大脑切片上所有主要神经柱的内部组织，使用针对突触素，血清素和？氨基丁酸特别强调的是描述太阳罗盘相关的大脑区域的神经结构，并概述了他们的同源性，以其他迁徙物种。除了发现与其他昆虫的许多一般解剖学相似之处外，物种间的比较还揭示了帝王蝶大脑特有的几个特征。这些独特的特征在视觉系统和蘑菇体中尤其明显。总的来说，我们提供了一个全面的分析，大脑解剖的帝王蝶，最终将帮助我们了解的神经元的过程，管理动物迁移。J.Comp.神经元520：15991628，2012。(C)2012 Wiley Periodicals，Inc.
Each fall, eastern North American monarch butterflies (Danaus plexippus) use a time-compensated sun compass to migrate to their overwintering grounds in central Mexico. The sun compass mechanism involves the neural integration of skylight cues with timing information from circadian clocks to maintain a constant heading. The neuronal substrates for the necessary interactions between compass neurons in the central complex, a prominent structure of the central brain, and circadian clocks are largely unknown. To begin to unravel these neural substrates, we performed 3D reconstructions of all neuropils of the monarch brain based on anti-synapsin labeling. Our work characterizes 21 well-defined neuropils (19 paired, 2 unpaired), as well as all synaptic regions between the more classically defined neuropils. We also studied the internal organization of all major neuropils on brain sections, using immunocytochemical stainings against synapsin, serotonin, and ?-aminobutyric acid. Special emphasis was placed on describing the neuroarchitecture of sun-compass-related brain regions and outlining their homologies to other migratory species. In addition to finding many general anatomical similarities to other insects, interspecies comparison also revealed several features that appear unique to the monarch brain. These distinctive features were especially apparent in the visual system and the mushroom body. Overall, we provide a comprehensive analysis of the brain anatomy of the monarch butterfly that will ultimately aid our understanding of the neuronal processes governing animal migration. J. Comp. Neurol. 520:15991628, 2012. (C) 2012 Wiley Periodicals, Inc.