Regulation of the high affinity receptor for IgE on human epidermal Langerhans cells.
Regulation of the high affinity receptor for IgE on human epidermal Langerhans cells.
复制标题
人表皮朗格汉斯细胞上 IgE 高亲和力受体的调节。
作者：
S. Kraft;J. H. Weßendorf;D. Hanau;T. Bieber
人表皮朗格汉斯细胞（LC）表达不同量的IgE高亲和力受体（Fc ε RI）;最强的表达是特应性皮炎的特征。该受体被认为是通过在IgE介导的迟发型超敏反应中作为空气过敏原和Ag特异性T细胞之间的联系参与这种疾病的病理生理学。在本研究中，我们表明，即使在没有表面表达的情况下，正常LC也保持相同分子量的Fc ε RI α链（Fc ε RI α）的细胞内库。作为表面结合的Fc ε RI α，其能够结合显著量的IgE。表面表达的缺乏与γ链（Fc ε RI γ）的缺乏或非常低的表达有关。此外，在细胞表面表达的Fc ε RI α的量与Fc ε RI γ的量显著相关。LC向淋巴样树突状细胞的分化伴随着Fc ε RI α转录物的消失，但不伴随Fc ε RI γ。这导致Fc ε RI α的细胞内和表面水平快速降低，而这不受IL-4、IgE或其他试剂的影响。总的来说，我们的研究结果表明，这些机制使LC成为高度通用的APC通过快速适应不同的炎症环境的Fc ε RI的表面水平。
Human epidermal Langerhans cells (LC) express variable amounts of the high affinity receptor for IgE (Fc epsilonRI); the strongest expression is characteristic of atopic dermatitis. The receptor is suggested to take part in the pathophysiology of this disease by acting as a link between aeroallergens and Ag-specific T cells in an IgE-mediated, delayed-type hypersensitivity reaction. In the present study we show that even in the absence of surface expression, normal LC maintain an intracellular pool of the alpha-chain of Fc epsilonRI (Fc epsilonRIalpha) of the same m.w. as the surface-bound Fc epsilonRIalpha that is able to bind significant amounts of IgE. The lack of surface expression is linked to the absence or very low expression of the gamma-chain (Fc epsilonRIgamma). Moreover, the amount of Fc epsilonRIalpha expressed at the cell surface significantly correlates with the amount of Fc epsilonRIgamma. LC differentiation toward lymphoid dendritic cells is accompanied by the disappearance of transcripts for Fc epsilonRIalpha, but not for Fc epsilonRIgamma. This leads to a rapid decrease in the intracellular and surface levels of Fc epsilonRIalpha, which cannot be influenced by IL-4, IgE, or other agents. Overall, our findings suggest that these mechanisms enable LC to be highly versatile APCs by rapidly adapting the surface level of Fc epsilonRI to distinct inflammatory environments.
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影响因子：
15.9
作者：
Dombrowicz, D;Flamand, V;Kinet, JP
通讯作者：
Kinet, JP
血清 IgE 水平驱动嗜碱性粒细胞和肥大细胞 IgE 受体显示。
影响因子：
2.8
作者：
MacGlashanJr,DW;Bochner,BS;Adelman,DC;Jardieu,PM;Togias,A;Lichtenstein,LM
通讯作者：
Lichtenstein,LM
转染细胞中突变 IgE 受体的固定和内化。
DOI：
--
发表时间：
1991
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Mao,SY;Varin-Blank,N;Edidin,M;Metzger,H
通讯作者：
Metzger,H
DOI：
10.1073/pnas.78.4.2541
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
FITHIAN, E;KUNG, P;EDELSON, R
通讯作者：
EDELSON, R