The effect of radiotherapy on NKT cells in patients with advanced head and neck cancer
The effect of radiotherapy on NKT cells in patients with advanced head and neck cancer
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DOI：
10.1007/s00262-010-0877-2
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发表时间：
2010-10-01
影响因子：
5.8
通讯作者：
Okamoto, Yoshitaka
中科院分区：
文献类型：
--
作者：
Kobayashi, Kouichi;Tanaka, Yuriko;Okamoto, Yoshitaka
用NKT细胞进行癌症免疫治疗是晚期头颈癌潜在的新治疗策略。NKT细胞疗法由于其独特的抗肿瘤活性和与当前疗法相比更高的安全性而具有前景。放射治疗作为晚期头颈部癌症的标准治疗是不可或缺的。为探讨NKT细胞作为放疗辅助免疫治疗的可能性，我们检测了12例IV期头颈部鳞癌患者放疗前后NKT细胞的数量、IFN-γ产生和增殖能力，并分析了放疗对NKT细胞的影响。体外检测NKT细胞的细胞毒活性，不同患者血液中NKT细胞的数量差异很大。放射治疗后，CD 3 T细胞群显著下降，而NKT细胞群保持稳定。放射治疗后NKT细胞数量与治疗前相同。放射治疗后立即收集的NKT细胞的IFN-γ产生在外源性配体刺激后受到损害，但与放射治疗前收集的细胞相比，这些NKT细胞的增殖反应增强。此外，增殖的NKT细胞显示出显着水平的抗肿瘤活性。NKT细胞对辐射具有相对抗性，因此可能适用于辅助免疫治疗，以消除接受过放射治疗的患者中的残留癌细胞。
Cancer immunotherapy with NKT cells is a potential new treatment strategy for advanced head and neck cancer. NKT cell therapy is promising due to its unique anti-tumor activity and higher degree of safety compared to current therapies. Radiotherapy is indispensable as a standard treatment for advanced head and neck cancer. To elucidate the possibility of using NKT cells as an adjuvant immunotherapy with radiotherapy, we examined the effect of radiotherapy on NKT cells in patients with head and neck cancer.The number, IFN-gamma production and proliferation capacity of NKT cells were analyzed before and after 50 Gy radiation therapy in 12 patients with stage IV head and neck squamous cell carcinoma. The cytotoxic activity of NKT cells was examined in vitro.The number of NKT cells in the blood varied widely between patients. After radiation therapy, the population of CD3 T cells decreased significantly, while the NKT cell population remained stable. The number of NKT cells was the same after radiation therapy as before. IFN-gamma production from NKT cells collected just after radiotherapy was impaired after stimulation with exogenous ligand, but the proliferative responses of these NKT cells was enhanced in comparison to those collected before radiation therapy. Furthermore, the proliferated NKT cells displayed a significant level of anti-tumor activity.NKT cells are relatively resistant to radiation and might therefore be suitable for adjuvant immunotherapy to eradicate remnant cancer cells in patients who have undergone radiation therapy.