N-to-C Sequential Ligation Using Peptidyl N,N-Bis(2-mercaptoethyl)amide Building Blocks
N-to-C Sequential Ligation Using Peptidyl N,N-Bis(2-mercaptoethyl)amide Building Blocks
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DOI：
10.1021/ol2031284
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发表时间：
2012-01-06
期刊：
影响因子：
5.2
通讯作者：
Liu, Chuan-Fa
中科院分区：
文献类型：
--
作者：
Yang, Renliang;Hou, Wen;Liu, Chuan-Fa
一种新的N-到C-末端顺序化学连接方法已被开发用于蛋白质合成。该策略的关键是N，N-双（2-巯基乙基）酰胺（BMEA）对天然化学连接的常规条件的相对稳定性。我们还发现了用于BMEA介导的连接反应的新的硫醇添加剂。这种方法的实用性在中等大小的肽和泛素的合成中得到了证明。
A novel N- to C-terminus sequential chemical ligation approach has been developed for protein synthesis. Key to this strategy is the relative stability of the N,N-bis(2-mercaptoethyl)amide (BMEA) to the conventional conditions of native chemical ligation. We have also found a new thiol additive for the BMEA-mediated ligation reaction. The usefulness of this approach was demonstrated in the syntheses of a medium-sized peptide and ubiquitin.