Rapid replacement of somatic linker histones with the oocyte-specific linker histone H1foo in nuclear transfer.
Rapid replacement of somatic linker histones with the oocyte-specific linker histone H1foo in nuclear transfer.
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DOI：
10.1016/j.ydbio.2003.10.004
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发表时间：
2004-02
影响因子：
2.7
通讯作者：
Takahide Teranishi;Mamoru Tanaka;Shingo Kimoto;Y. Ono;K. Miyakoshi;T. Kono;Y. Yoshimura
中科院分区：
文献类型：
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作者：
Takahide Teranishi;Mamoru Tanaka;Shingo Kimoto;Y. Ono;K. Miyakoshi;T. Kono;Y. Yoshimura
卵母细胞特异性连接组蛋白最显著的特征是其在胚胎发育过程中表达的特定时间。在非洲爪蟾核移植中，供体核中的体细胞连接组蛋白被卵母细胞特异性连接组蛋白B4取代，导致卵母细胞特异性连接组蛋白参与核重编程。我们最近发现了一个小鼠卵母细胞特异性连接组蛋白，命名为H1foo，并证明了其在正常植入前胚胎中的表达模式。本研究旨在确定在小鼠核移植过程中是否发生体细胞连接组蛋白与H1foo的替换。H1foo在移植后不久在供核中被检测到。此后，H1foo仅限于两细胞期胚胎的染色质。在将卵母细胞与表达GFP（绿色荧光蛋白）标记的体细胞接头组蛋白H1c的细胞融合后，观察到供体核中H1c的立即释放。此外，我们使用荧光恢复后的光漂白（FRAP），并发现H1foo是更移动的比H1c在活细胞。H1foo的更大的移动性可能有助于其快速替换和胚胎染色质结构的稳定性降低。这些结果表明，H1c与H1foo的快速替换可能在核重塑中发挥重要作用。
The most distinctive feature of oocyte-specific linker histones is the specific timing of their expression during embryonic development. In Xenopus nuclear transfer, somatic linker histones in the donor nucleus are replaced with oocyte-specific linker histone B4, leading to the involvement of oocyte-specific linker histones in nuclear reprogramming. We recently have discovered a mouse oocyte-specific linker histone, named H1foo, and demonstrated its expression pattern in normal preimplantation embryos. The present study was undertaken to determine whether the replacement of somatic linker histones with H1foo occurs during the process of mouse nuclear transfer. H1foo was detected in the donor nucleus soon after transplantation. Thereafter, H1foo was restricted to the chromatin in up to two-cell stage embryos. After fusion of an oocyte with a cell expressing GFP (green fluorescent protein)-tagged somatic linker histone H1c, immediate release of H1c in the donor nucleus was observed. In addition, we used fluorescence recovery after photobleaching (FRAP), and found that H1foo is more mobile than H1c in living cells. The greater mobility of H1foo may contribute to its rapid replacement and decreased stability of the embryonic chromatin structure. These results suggest that rapid replacement of H1c with H1foo may play an important role in nuclear remodeling.