Characterisation of the Escherichia coli mfd promoter
Characterisation of the Escherichia coli mfd promoter
复制标题
大肠杆菌 mfd 启动子的表征
作者：
L. Stanley;N. Savery
细菌mfd基因编码一种转录修复偶联因子，介导DNA损伤在活性转录单位的模板链中的优先修复。本研究确定了大肠杆菌mfd基因在体内和体外的转录起始点，确定了mfd基因缺失和定点突变转录的DNA决定因素。依赖于σ70的典型启动子mfd P1负责mfd转录的大部分，而由−42到+5残基组成的核心区足以在丰富和最少的介质中发挥全部活性。
The bacterial mfd gene encodes a transcription-repair coupling factor that mediates the preferential repair of DNA damage in the template strand of active transcriptional units. In this report, the transcription start site for the Escherichia coli mfd gene was determined in vivo and in vitro, and the DNA determinants for mfd transcription by deletion and site-directed mutagenesis were defined. A canonical σ70-dependent promoter, mfd P1, was responsible for the majority of mfd transcription, and a core region consisting of residues −42 to +5 was sufficient for full activity in rich and minimal media.
影响因子：
3.9
作者：
Tornaletti, S;Hanawalt, PC
通讯作者：
Hanawalt, PC
影响因子：
14.9
作者：
McCue, LA;Thompson, W;Lawrence, CE
通讯作者：
Lawrence, CE