Engineering anatomically shaped vascularized bone grafts with hASCs and 3D-printed PCL scaffolds
Engineering anatomically shaped vascularized bone grafts with hASCs and 3D-printed PCL scaffolds
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DOI：
10.1002/jbm.a.35107
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发表时间：
2014-12-01
影响因子：
4.9
通讯作者：
Grayson, Warren L.
中科院分区：
文献类型：
--
作者：
Temple, Joshua P.;Hutton, Daphne L.;Grayson, Warren L.
由于可移植自体骨移植的可用性有限和骨的复杂几何形状，治疗颅颌面骨缺损在临床上具有挑战性。再生能够忠实地复制解剖结构的新骨组织的能力将彻底改变治疗选择。在过去的几十年里，骨组织工程领域的进展为使用生物相容性支架材料和自体细胞提供了有前途的新治疗方案。这种方法与最新的三维（3D）打印技术相结合，可能很快就会产生具有定制的、患者特定结构的大型生物人工骨移植物。在这项研究中，我们使用定制的3D打印机来开发具有不同内部孔隙率的解剖形状的聚己内酯（PCL）支架。这些支架被评估其支持诱导人类脂肪源性干细胞（hASCs）形成血管和骨骼的能力，这是功能性骨组织的两个基本组成部分。在体外和体内评估功能组织的发育。最后，我们展示了打印大型下颌和上颌骨支架的能力，该支架可以复制从患者计算机断层扫描中提取的精细细节。这项研究的发现说明了3D打印支架用于工程自体、解剖形状、血管化骨移植的能力和潜力。(c) 2014 Wiley期刊公司[J]中国生物医学工程学报，2014,31(2):557 - 557。
The treatment of large craniomaxillofacial bone defects is clinically challenging due to the limited availability of transplantable autologous bone grafts and the complex geometry of the bones. The ability to regenerate new bone tissues that faithfully replicate the anatomy would revolutionize treatment options. Advances in the field of bone tissue engineering over the past few decades offer promising new treatment alternatives using biocompatible scaffold materials and autologous cells. This approach combined with recent advances in three-dimensional (3D) printing technologies may soon allow the generation of large, bioartificial bone grafts with custom, patient-specific architecture. In this study, we use a custom-built 3D printer to develop anatomically shaped polycaprolactone (PCL) scaffolds with varying internal porosities. These scaffolds are assessed for their ability to support induction of human adipose-derived stem cells (hASCs) to form vasculature and bone, two essential components of functional bone tissue. The development of functional tissues is assessed in vitro and in vivo. Finally, we demonstrate the ability to print large mandibular and maxillary bone scaffolds that replicate fine details extracted from patient's computed tomography scans. The findings of this study illustrate the capabilities and potential of 3D printed scaffolds to be used for engineering autologous, anatomically shaped, vascularized bone grafts. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 4317-4325, 2014.