Superoxide radical scavenging activity of the major polyphenols in fresh plums
Superoxide radical scavenging activity of the major polyphenols in fresh plums
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DOI：
10.1021/jf034740d
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发表时间：
2003-12-31
影响因子：
6.1
通讯作者：
Lee, CY
中科院分区：
文献类型：
--
作者：
Chun, OK;Kim, DO;Lee, CY
采用酶法研究了不同品种李多酚对超氧阴离子自由基清除活性的影响，并测定了其IC 50值。我们发现多酚的SRSA水平与其化学结构密切相关;矢车菊素在所检测的多酚中显示出最低的IC 50，并且苷元比其糖苷更有效。BY 69-339在11个李品种中表现出最低的IC 50，这意味着清除超氧自由基的抗氧化活性最高，其次是French Damson、Cacaks Best、贝尔茨维尔Elite 870197、皇后、卡斯尔顿、斯坦利、NY 6、NY 101、Mirabellier和NY 9。IC_（50）值与总黄酮的相关性（r（2）= 0.8699）高于与总酚的相关性（r（2）= 0.8355），表明黄酮类化合物对总SRSA的贡献可能比其它多酚类化合物更直接。除了两个黄色品种不含花色苷外，花色苷是李子总SRSA的主要贡献者。绿原酸是最主要的酚酸，在1.0 ~ 94.9%范围内也表现出显著的SRSA。栎精是李中主要的黄酮醇。然而，它们对整个撒哈拉以南非洲的贡献相对较低。
The effect of polyphenols among different varieties of plums on superoxide radical scavenging activity (SRSA) was studied by an enzymatic method and their IC50 values were determined. We found that the SRSA levels of the polyphenols were closely related to their chemical structures; cyanidin showed the lowest IC50 among the polyphenols examined, and aglycones are more effective than their glycosides. BY 69-339 cultivar exhibited the lowest IC50 among the eleven plum cultivars, which means the highest antioxidant activity in scavenging superoxide radicals, followed by French Damson, Cacaks Best, Beltsville Elite 870197, Empress, Castleton, Stanley, NY 6, NY 101, Mirabellier, and NY 9. IC50 values showed a higher correlation with total flavonoids (r(2) = 0.8699) than total phenolics (r(2) = 0.8355), which indicated that flavonoids might contribute to the total SRSA more directly than other polyphenols. Anthocyanins in plums appeared to be the major contributors to the total SRSA, except for two yellow cultivars having no anthocyanins. Chlorogenic acid was the predominant phenolic acid, and it also exhibited SRSA significantly in the range of 1.0 to 94.9%. Quercetins were the major flavonols in plums. However, they showed relatively low contribution to the total SRSA.