Comparison of various process engineering concepts of preparative chromatography
Comparison of various process engineering concepts of preparative chromatography
复制标题
制备色谱各种工艺工程概念的比较
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
A. Seidel
中科院分区：
文献类型：
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作者：
C. Heuer;H. Kniep;T. Falk;A. Seidel
利用多孔固相的液相色谱（吸附色谱）是现代分析化学中最强大和最通用的方法之一。其广泛应用部分归因于固体固定相和液体移动的相的广泛组合的开发。分别使用纯的和化学改性的二氧化硅的正相和反相色谱法，亲和色谱法，可以被引用作为实例。除了用作分析方法外，色谱法在制药工业、生物技术和精细化学品生产中用于分离纯物质的制备规模中变得越来越重要。在许多情况下，所需的纯度标准只能通过色谱技术来满足，如在外消旋体的拆分中。从工艺工程的角度来看，最近已经获得了由分离柱的预期过载引起的浓度分布变形的更好的理解。特别是，这涉及到与竞争吸附相关的置换和夹带效应的描述。开发了一系列可供选择的操作模式，并用于提高产量和生产率。尽管一些尝试比较研究，定量信息优化这些过程是缺乏的。本文将在统一理论模型的基础上，比较四种不同的制备色谱过程工程概念。
Liquid chromatography utilizing porous solid phases (adsorption chromatography) is one of the most powerful and versatile methods of modern analytical chemistry. Its widespread application is due in part to the development of a broad range of combinations of solid stationary and liquid mobile phases. Normal phase and reverse phase chromatography with pure and chemically modified silica, respectively, affinity chromatography, can be cited as examples. In addition to its use as an analytical method, chromatography is becoming increasingly important in the preparative scale for the isolation of pure substances in the pharmaceutical industry, in biotechnology, and in the production of fine chemicals. In many cases, the required purity criteria can only be met with chromatographic techniques, as in the resolution of racemates. From a process engineering viewpoint, a better understanding of concentration profile deformations caused by intended overloading of separation columns has recently been acquired. In particular, this concerns the description of displacement and entrainment effects associated with the competitive adsorption. A series of alternative operating modes has been developed and used to enhance yields and productivities. In spite of a number of attempted comparative studies, quantitative information about optimizing these processes is lacking. This article will compare four different process engineering concepts of preparative chromatography on the basis of a uniform theoretical model.