INFLUENCE OF NUCLEAR BACKGROUND ON TRANSCRIPTION OF A CHIMERIC GENE (ORF256) AND COXI IN FERTILE AND CYTOPLASMIC MALE-STERILE WHEATS
INFLUENCE OF NUCLEAR BACKGROUND ON TRANSCRIPTION OF A CHIMERIC GENE (ORF256) AND COXI IN FERTILE AND CYTOPLASMIC MALE-STERILE WHEATS
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DOI：
10.1139/g94-028
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发表时间：
1994-04-01
期刊：
影响因子：
3.1
通讯作者：
HEDGCOTH, C
中科院分区：
文献类型：
--
作者：
SONG, JS;HEDGCOTH, C
作为母本供体的蒂莫菲维小麦与普通小麦之间的杂交可以产生细胞质雄性不育（cms）植物。 T. timopheevi 源自亲本、cms 和育性恢复系的线粒体 DNA 与源自 I aestivum 的线粒体 DNA 的 coxI 基因区域不同。我们之前对 ems 品系的结果表明，在 T. timopheevi 衍生的 mtDNA 中，在 coxI 的上游存在一个开放阅读框 orf256，而在普通小麦 DNA 中不存在。 orf256编码区的5'侧翼区和前33个核苷酸与普通小麦coxI的相应区域相同，而orf256的其余部分，包括3'侧翼，与coxI无关。此外，来自蒂莫菲维 (T. timopheevi) 衍生 mtDNA 的 HindIII 片段上的 orf256 和 coxI 的组织在蒂莫菲维 (T. timopheevi)、ems 和育性恢复系中是相同的。我们现在报道，orf256 的 DNA 序列在 T. timopheevi、ems 和育性恢复品系中是相同的。 ems和育性恢复品系中的主要转录物编码orf256和具有5'末端的coxI，如普通小麦的coxI mRNA，而来自蒂莫菲维的亲本线粒体具有在orf256编码区内具有5'末端的主要转录物。来自 ems 和生育力恢复品系的线粒体有可能产生亲本 T. timopheevi 或 T. aestivum 中不存在的蛋白质。
Crosses between Triticum timopheevi, as maternal donor, and T. aestivum can lead to cytoplasmic male sterile (cms) plants. The T. timopheevi derived mitochondrial DNA from parental, cms, and fertility-restored lines differs from that of I aestivum derived mtDNA in the coxI gene region. Our previous results for ems lines showed that there is an open reading frame, orf256, upstream from coxI in T. timopheevi derived mtDNA that is not present in T. aestivum DNA. The 5' flanking region and the first 33 nucleotides of the coding region of orf256 are identical to the corresponding region of T. aestivum coxI, whereas the rest of orf256, including the 3' flank, is not related to coxI. Also, the organization of orf256 and coxI on a HindIII fragment from T. timopheevi derived mtDNA are identical in T. timopheevi, ems, and fertility-restored lines. We now report that the DNA sequence of orf256 is identical in T. timopheevi, ems, and fertility-restored lines. Major transcripts in ems and fertility-restored lines encode both orf256 and coxI with 5' termini like coxI mRNA of T. aestivum, whereas parental mitochondria from T. timopheevi have major transcripts with 5' termini within the orf256 coding region. Mitochondria from ems and fertility-restored lines have the potential to produce a protein that would not be present in parental T. timopheevi or in T. aestivum.