TRH microdialysis into the RTN of the conscious rat increases breathing, metabolism, and temperature
TRH microdialysis into the RTN of the conscious rat increases breathing, metabolism, and temperature
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DOI：
10.1152/jappl.1999.87.2.673
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发表时间：
1999-08-01
影响因子：
3.3
通讯作者：
Li, AH
中科院分区：
文献类型：
--
作者：
Cream, C;Nattie, E;Li, AH
将促甲状腺激素释放激素(TRH)注射到麻醉大鼠的梯形后核(RTN)中，产生大量、长时间的通气输出刺激(C.L.Cream、A.Li和E.E.Nattie.J.Appl.Physiol.83：792-799，1997)。在这里，我们将 TRH 注射或透析到清醒大鼠的 RTN 中。在 17 次注射中的 6 次（200 nl，3.1 +/- 1.7 mM）中，通气量（(V) 相对于 E 点）在 10 分钟内增加了 31%，并在 60 分钟内恢复。通过透析，一组（n = 5）的每只动物以随机顺序接受 10 mM TRH、10 mM TRHOH（TRH 的代谢物）和人工脑脊液 (aCSF)；第二组 (n = 5) 的每只动物接受 aCSF 和 1 mM TRH。 TRHOH 和 aCSF 没有持续影响。 TRH (1 mM) 比点 E 增加 (V)（32%，10 分钟后 P < 0.02，60 分钟恢复）、Oz 消耗量（(V) 比点 o(2)；19%，P < 0.03）和体（直肠）温度 (T-re；0.5 摄氏度，(P < 0.09)。TRH (10 mM) 比点 E 增加 (V) （78% P < 0.01，10 分钟后，60 分钟后没有恢复），(V) 点 O-2（48%，P < 0.01）和 T-re（1.0 摄氏度，P < 0.01）也诱导唤醒。通过透析荧光素的扩散（332.3 mol wt，mol wt）来估计透析中受影响的组织体积。 TRH = 362.4)，10 mM (n = 5) 为 1,580 +/- 256 nl，1 mM (n = 5) 为 590 +/- 128 nl，我们得出结论：1) RTN 参与 (V) 点 E、(V) 点 O-2、T-re 和唤醒的整合，2) TRH 可能确定 RTN 的响应性。神经元。
Thyrotropin-releasing hormone (TRH) injected into the retrotrapezoid nucleus (RTN) of anesthetized rats produces a large, prolonged stimulation of ventilatory output (C. L. Cream, A. Li, and E. E. Nattie. J. Appl. Physiol. 83: 792-799, 1997). Here we inject or dialyze TRH into the RTN of conscious rats. In 6 of 17 injections (200 nl, 3.1 +/- 1.7 mM), ventilation ((V) over dot E) increased 31% by 10 min, with recovery by 60 min. With dialysis, each animal of one group (n = 5) received, in random order, 10 mM TRH, 10 mM TRHOH (a metabolite of TRH), and artificial cerebrospinal fluid (aCSF); each animal of a second group (n = 5) received aCSF and 1 mM TRH. TRHOH and aCSF had no sustained effects. TRH (1 mM) increased (V) over dot E (32%, P < 0.02 by 10 min, with recovery by 60 min), Oz consumption ((V) over dot o(2); 19%, P < 0.03), and body (rectal) temperature (T-re; 0.5 degrees C, (P < 0.09). TRH (10 mM) increased (V) over dot E (78% P < 0.01, by 10 min, with no recovery at 60 min), (V) over dot O-2 (48%, P < 0.01), and T-re (1.0 degrees C, P < 0.01). TRH also induced arousal. The tissue volume affected in dialysis, estimated by spread of dialyzed fluorescein (332.3 mol wt, mol wt of TRH = 362.4), was 1,580 +/- 256 nl for 10 mM (n = 5) and 590 +/- 128 nl for 1 mM (n = 5). We conclude that 1) the RTN is involved in the integration of (V) over dot E, (V) over dot O-2, T-re, and arousal and 2) TRH may establish the responsiveness of RTN neurons.