Genetic and Biochemical Characterization of a Pathway for the Degradation of 2-Aminoethylphosphonate in Sinorhizobium meliloti 1021
Genetic and Biochemical Characterization of a Pathway for the Degradation of 2-Aminoethylphosphonate in Sinorhizobium meliloti 1021
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DOI：
10.1074/jbc.m111.237735
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发表时间：
2011-06-24
影响因子：
4.8
通讯作者：
Metcalf, William W.
中科院分区：
文献类型：
--
作者：
Borisova, Svetlana A.;Christman, Harry D.;Metcalf, William W.
多种微生物具有使用膦酸作为磷的唯一来源的能力。在此，基于基因组序列分析，提出了苜蓿中华根瘤菌1021降解2-氨基乙基膦酸酯的新途径。基因缺失实验证实了包含phnW、phnA和phnY基因的位点参与2-氨基乙基膦酸盐向无机磷酸盐的转化。重组PhnY和PhnA蛋白的生化研究验证了其作为膦酰乙醛脱氢酶和膦酰乙酸水解酶的作用，分别。这种途径可能不仅限于S。在其他细菌基因组中存在同源基因簇，这表明苜蓿草属是一种细菌。
A variety of microorganisms have the ability to use phosphonic acids as sole sources of phosphorus. Here, a novel pathway for degradation of 2-aminoethylphosphonate in the bacterium Sinorhizobium meliloti 1021 is proposed based on the analysis of the genome sequence. Gene deletion experiments confirmed the involvement of the locus containing phnW, phnA, and phnY genes in the conversion of 2-aminoethylphosphonate to inorganic phosphate. Biochemical studies of the recombinant PhnY and PhnA proteins verified their roles as phosphonoacetaldehyde dehydrogenase and phosphonoacetate hydrolase, respectively. This pathway is likely not limited to S. meliloti as suggested by the presence of homologous gene clusters in other bacterial genomes.