Transcriptome-Wide Identification of 2'-O-Methylation Sites with RibOxi-Seq.
Transcriptome-Wide Identification of 2'-O-Methylation Sites with RibOxi-Seq.
复制标题
DOI：
10.1007/978-1-0716-1851-6_22
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
以高通量方式检测2 ' - o -甲基化位点（Nm）的能力是重要的，因为越来越多的证据表明这种RNA修饰的前景更加多样化，以及尚未确定功能的可能性。在这里，我们描述了一个优化版本的RibOxi-seq，这是建立在最初发表的方法，不仅准确地描绘核糖体RNA （rRNA）纳米位点，最小的RNA输入，而且还足够强大，以识别mRNA的内含子和外显子位点。
The ability to detect 2′-O-methylation sites (Nm) in high-throughput fashion is important, as increasing evidence points to a more diverse landscape for this RNA modification as well as the possibility of yet unidentified functions. Here we describe an optimized version of RibOxi-seq, which is built upon the original published method, that not only accurately profiles ribosomal RNA (rRNA) Nm sites with minimal RNA input but is also robust enough to identify mRNA intronic and exonic sites.