Delivery of functional exogenous proteins by plant-derived vesicles to human cells in vitro.
Delivery of functional exogenous proteins by plant-derived vesicles to human cells in vitro.
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DOI：
10.1038/s41598-021-85833-y
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发表时间：
2021-03-22
影响因子：
4.6
通讯作者：
Shtam T
中科院分区：
文献类型：
--
作者：
Garaeva L;Kamyshinsky R;Kil Y;Varfolomeeva E;Verlov N;Komarova E;Garmay Y;Landa S;Burdakov V;Myasnikov A;Vinnikov IA;Margulis B;Guzhova I;Kagansky A;Konevega AL;Shtam T
Plant-derived extracellular vesicles (EVs) gain more and more attention as promising carriers of exogenous bioactive molecules to the human cells. Derived from various edible sources, these EVs are remarkably biocompatible, biodegradable and highly abundant from plants. In this work, EVs from grapefruit juice were isolated by differential centrifugation followed by characterization of their size, quantity and morphology by nanoparticle tracking analysis, dynamic light scattering, atomic force microscopy and cryo-electron microscopy (Cryo-EM). In Cryo-EM experiments, we visualized grapefruit EVs with the average size of 41 ± 13 nm, confirmed their round-shaped morphology and estimated the thickness of their lipid bilayer as 5.3 ± 0.8 nm. Further, using cell culture models, we have successfully demonstrated that native grapefruit-derived extracellular vesicles (GF-EVs) are highly efficient carriers for the delivery of the exogenous Alexa Fluor 647 labeled bovine serum albumin (BSA) and heat shock protein 70 (HSP70) into both human peripheral blood mononuclear cells and colon cancer cells. Interestingly, loading to plant EVs significantly ameliorated the uptake of exogenous proteins by human cells compared to the same proteins without EVs. Most importantly, we have confirmed the functional activity of human recombinant HSP70 in the colon cancer cell culture upon delivery by GF-EVs. Analysis of the biodistribution of GF-EVs loaded with 125I-labeled BSA in mice demonstrated a significant uptake of the grapefruit-derived extracellular vesicles by the majority of organs. The results of our study indicate that native plant EVs might be safe and effective carriers of exogenous proteins into human cells.
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影响因子：
5.6
作者：
Gangadaran P;Hong CM;Oh JM;Rajendran RL;Kalimuthu S;Son SH;Gopal A;Zhu L;Baek SH;Jeong SY;Lee SW;Lee J;Ahn BC
通讯作者：
Ahn BC
外泌体作为帕金森氏病疗法的药物。
DOI：
10.1016/j.jconrel.2015.03.033
发表时间：
2015-06-10
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Haney MJ;Klyachko NL;Zhao Y;Gupta R;Plotnikova EG;He Z;Patel T;Piroyan A;Sokolsky M;Kabanov AV;Batrakova EV
通讯作者：
Batrakova EV
影响因子：
4.6
作者：
Li Z;Wang H;Yin H;Bennett C;Zhang HG;Guo P
通讯作者：
Guo P
影响因子：
1.2
作者：
Marimuthu, Rekha;Francis, Habib;Williams, Helen
通讯作者：
Williams, Helen
影响因子：
3.7
作者：
Issman, Liron;Brenner, Benjamin;Aharon, Anat
通讯作者：
Aharon, Anat