Genome-wide transcription profiling of the early phase of Biofilm formation by Candida albicans
Genome-wide transcription profiling of the early phase of Biofilm formation by Candida albicans
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DOI：
10.1128/ec.4.9.1562-1573.2005
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发表时间：
2005-09-01
期刊：
影响因子：
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通讯作者：
Agabian, NM
中科院分区：
文献类型：
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作者：
Murillo, LA;Newport, G;Agabian, NM
附着在表面并发展成多细胞群落的能力是大多数微生物在不断变化的环境中生存所使用的适应能力。生物膜的形成经历了不同的发育阶段，不仅影响医学，也影响工业和进化。在机会性病原体白色念珠菌等生物体中，作为生物膜生长的能力也是持久性的重要机制，促进其在不同组织和医疗器械中使用的广泛的非生物表面上生长。白色念珠菌生物膜的早期特征是单细胞与基质的粘附，随后形成复杂的菌丝网络并开始致密结构。转录组在与底物接触30分钟内开始发生变化，包括硫代谢相关基因的表达，特别是MET3，以及酿酒酵母中Ribi调控的等效基因同源物。其中一些更改在早期启动并在整个过程中保持；另一些则局限于生物膜形成的早期阶段。我们在此确定了白色念珠菌中参与谷胱甘肽产生的半胱氨酸代谢和生物膜相关基因表达的潜在替代途径。
The ability to adhere to surfaces and develop as a multicellular community is an adaptation used by most microorganisms to survive in changing environments. Biofilm formation proceeds through distinct developmental phases and impacts not only medicine but also industry and evolution. In organisms such as the opportunistic pathogen Candida albicans, the ability to grow as biofilms is also an important mechanism for persistence, facilitating its growth on different tissues and a broad range of abiotic surfaces used in medical devices. The early stage of C. albicans biofilm is characterized by the adhesion of single cells to the substratum, followed by the formation of an intricate network of hyphae and the beginning of a dense structure. Changes in the transcriptome begin within 30 min of contact with the substrate and include expression of genes related to sulfur metabolism, in particular MET3, and the equivalent gene homologues of the Ribi regulon in Saccharomyces cerevisiae. Some of these changes are initiated early and maintained throughout the process; others are restricted to the earliest stages of biofilm formation. We identify here a potential alternative pathway for cysteine metabolism and the biofilm-associated expression of genes involved in glutathione production in C. albicans.