Pigment Pattern Formation by Contact-Dependent Depolarization
Pigment Pattern Formation by Contact-Dependent Depolarization
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DOI：
10.1126/science.1212821
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发表时间：
2012-02-10
期刊：
影响因子：
56.9
通讯作者：
Kondo, Shigeru
中科院分区：
文献类型：
--
作者：
Inaba, Masafumi;Yamanaka, Hiroaki;Kondo, Shigeru
尽管最近的实验研究表明，色素细胞之间的相互作用在皮肤图案的形成中发挥了关键作用，但其机制的细节仍很大程度上仍不清楚。通过使用体外细胞培养系统，我们检测到斑马鱼皮肤中两种色素细胞类型--黑素细胞和黄色素细胞之间的相互作用。在原代培养过程中，当黑素细胞膜与叶黄团的树突接触时，黑素细胞膜瞬间去极化。这种去极化触发了黑素载体的迁移，以避免与黄色素载体进一步接触。在含有美洲虎突变体的色素细胞中，没有观察到细胞去极化和排斥性运动，这表明黑色素和黄色素分离有缺陷。野生型色素细胞的去极化-排斥性可能解释了斑马鱼产生条纹的色素细胞行为。
Although recent experimental studies have suggested that the interactions among the pigment cells play a key role in the skin pattern formation, details of the mechanism remain largely unknown. By using an in vitro cell culture system, we have detected interactions between the two pigment cell types, melanophores and xanthophores, in the zebrafish skin. During primary culture, the melanophore membrane transiently depolarizes when contacted with the dendrites of a xanthophore. This depolarization triggers melanophore migration to avoid further contact with the xanthophores. Cell depolarization and repulsive movement were not observed in pigment cells with thejaguarmutant, which shows defective segregation of melanophores and xanthophores. The depolarization-repulsion of wild-type pigment cells may explain the pigment cell behaviors generating the stripe pattern of zebrafish.