Dual-channel-coded microbeads for multiplexed detection of biomolecules using assembling of quantum dots and element coding nanoparticles
Dual-channel-coded microbeads for multiplexed detection of biomolecules using assembling of quantum dots and element coding nanoparticles
复制标题
使用量子点和元素编码纳米粒子组装进行生物分子多重检测的双通道编码微珠
DOI：
10.1016/j.aca.2018.03.025
复制
发表时间：
2018
影响因子：
6.2
通讯作者：
Ji Yanhong
中科院分区：
文献类型：
--
作者：
Lu Bangrong;He Qinghua;He Yonghong;Chen Xuejing;Feng Guangxia;Liu Siyu;Ji Yanhong
To achieve the dual-channel (analog and digital) encoding, microbeads assembled with quantum dots (QDs) and element coding nanoparticles (ECNPs) have been prepared. Dual-spectra, including fluorescence generated from quantum dots (QDs) and laser induced breakdown spectrum obtained from the plasma of ECNPs, including AgO, MgO and ZnO nanoparticles, has been adopted to provide more encoding amounts and more accurate dual recognition for encoded microbeads in multiplexed utilization. The experimental results demonstrate that the single microbead can be decoded in two optical channels. Multiplexed analysis and contrast adsorption experiment of anti-IgG verified the availability and specificity of dual-channel-coded microbeads in bioanalysis. In gradient detection of anti-IgG, we obtained the linear concentration response to target biomolecules from 3.125 × 10−10M to 1 × 10−8M, and the limit of detection was calculated to be 2.91 × 10−11M.
