Notch signaling controls lineage specification during Drosophila larval hematopoiesis
Notch signaling controls lineage specification during Drosophila larval hematopoiesis
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DOI：
10.1016/s0960-9822(02)01297-6
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发表时间：
2002-11-19
期刊：
影响因子：
9.2
通讯作者：
Royet, J
中科院分区：
文献类型：
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作者：
Duvic, B;Hoffmann, JA;Royet, J
果蝇幼虫的血细胞来源于一种叫做淋巴腺的造血器官，淋巴腺由位于沿着背侧血管的成对叶组成。在循环血淋巴中发现两种成熟的血细胞群：巨噬细胞样浆细胞和含有免疫相关黑化过程酶的晶细胞。第三类细胞，称为板细胞，通常在幼虫中不存在，但在被太大而不能被吞噬的寄生虫感染后分化。在这里，我们提出的证据表明，Notch信号通路在晶体细胞的分化中起着指导作用。Notch的功能丧失突变导致晶体细胞数量严重减少，而Notch的过表达引起大量这些细胞的分化。我们证明，在这个过程中，锯齿，而不是三角洲，是Notch配体。此外，Notch功能对于寄生后的板层细胞增殖是必需的，尽管Notch过表达不会导致板层细胞产生。最后，Notch似乎在浆细胞谱系的分化中不起作用。这项研究强调了果蝇和哺乳动物造血的遗传控制存在相似之处。
Drosophila larval hemocytes originate from a hematopoietic organ called lymph glands, which are composed of paired lobes located along the dorsal vessel. Two mature blood cell populations are found in the circulating hemolymph: the macrophage-like plasmatocytes, and the crystal cells that contain enzymes of the immune-related melanization process. A third class of cells, called lamellocytes, are normally absent in larvae but differentiate after infection by parasites too large to be phagocytosed. Here we present evidence that the Notch signaling pathway plays an instructive role in the differentiation of crystal cells. Loss-of-function mutations in Notch result in severely decreased crystal cell numbers, whereas overexpression of Notch provokes the differentiation of high numbers of these cells. We demonstrate that, in this process, Serrate, not Delta, is the Notch ligand. In addition, Notch function is necessary for lamellocyte proliferation upon parasitization, although Notch overexpression does not result in lamellocyte production. Finally, Notch does not appear to play a role in the differentiation of the plasmatocyte lineage. This study underlines the existence of parallels in the genetic control of hematopoiesis in Drosophila and in mammals.