Anticancer Action of Xiaoxianxiong Tang in Non-Small Cell Lung Cancer by Pharmacological Analysis and Experimental Validation.
Anticancer Action of Xiaoxianxiong Tang in Non-Small Cell Lung Cancer by Pharmacological Analysis and Experimental Validation.
复制标题
小仙芎汤抗非小细胞肺癌的药理分析及实验验证
DOI：
10.1155/2021/9930082
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Bi L
中科院分区：
文献类型：
--
作者：
Ding R;Jiao L;Sang S;Yin Y;Wang Y;Gong Y;Xu L;Bi L
小仙芎汤（XXXT）是著名的中药配方。越来越多的证据支持 XXXT 在改善非小细胞肺癌 (NSCLC) 治疗方面的益处。本研究的目的旨在通过网络药理学分析和生物学验证探讨XXXT的作用和机制。 TCMSP数据库用于识别XXXT中的潜在活性化合物，包括吸收、分布、代谢、排泄筛选及其潜在靶标。通过搜索Therapeutic Target数据库、GeneCards数据库、DrugBank数据库、DisGeNET数据库预测与NSCLC相关的疾病靶点。在 4385 个 NSCLC 相关靶点中，156 个靶点也是 XXXT 中存在的化合物的靶点。随后，GO功能和KEGG通路富集以及PPI网络分析显示，XXXT中20种成分影响的95个靶点和20条通路中，20个靶点与患者生存相关，并且XXXT可以对PI3K-AKT信号通路发挥抑制作用。此外，XXXT以浓度依赖性方式抑制A549和H460 细胞的增殖，并抑制与PI3K-AKT通路密切相关的关键靶标CCNA2、FOSL2和BIRC5的mRNA和蛋白水平。因此，XXXT 有潜力通过靶向 PI3K-AKT 信号通路来改善 NSCLC 的治疗。
Xiaoxianxiong Tang (XXXT) is a well-known traditional Chinese medicine formula. Evidence is emerging supporting the benefits of XXXT in ameliorating therapy for non-small cell lung cancer (NSCLC). The purpose of this study aimed to explore the effects and mechanisms of XXXT through network pharmacological analysis and biological validation. TCMSP database was used to identify potentially active compounds in XXXT with absorption, distribution, metabolism, excretion screening, and their potential targets. The disease targets related to NSCLC were predicted by searching for Therapeutic Target database, GeneCards database, DrugBank database, and DisGeNET database. Of the 4385 NSCLC-related targets, 156 targets were also the targets of compounds present in XXXT. Subsequently, GO function and KEGG pathway enrichment and PPI network analyses revealed that, of the 95 targets and 20 pathways influenced by 20 ingredients in XXXT, 20 targets were associated with patient survival, and XXXT could exert an inhibitory action on the PI3K-AKT signaling pathway. Moreover, XXXT restrained the proliferation of A549 and H460 cells in a concentration-dependent manner and suppressed the mRNA and protein levels of key targets CCNA2, FOSL2, and BIRC5 closely linked to the PI3K-AKT pathway. Hence, XXXT has the potential to improve therapy for NSCLC by targeting the PI3K-AKT signaling pathway.
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影响因子：
254.7
作者：
Miller, Kimberly D.;Fidler-Benaoudia, Miranda;Siegel, Rebecca L.
通讯作者：
Siegel, Rebecca L.
影响因子：
13.6
作者：
Sarode, Poonam;Zheng, Xiang;Savai, Rajkumar
通讯作者：
Savai, Rajkumar
影响因子：
3.6
作者：
Li, Fengbo;Gu, Fengming;Zhu, Xuezhuan
通讯作者：
Zhu, Xuezhuan
影响因子：
7.5
作者：
Li，Guanting;Wu，Xianwei;Huang，Hongliang
通讯作者：
Huang，Hongliang
影响因子：
16.6
作者：
Napoli M;Li X;Ackerman HD;Deshpande AA;Barannikov I;Pisegna MA;Bedrosian I;Mitsch J;Quinlan P;Thompson A;Rajapakshe K;Coarfa C;Gunaratne PH;Marchion DC;Magliocco AM;Tsai KY;Flores ER
通讯作者：
Flores ER