SEROPREVALENCE OF LYME DISEASE IN EASTERN SLOVAKIA
SEROPREVALENCE OF LYME DISEASE IN EASTERN SLOVAKIA
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DOI：
10.21101/cejph.a5442
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发表时间：
2018-12-01
影响因子：
1.2
通讯作者：
Benhatchi, Karim
中科院分区：
文献类型：
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作者：
Busova, Andrea;Dorko, Erik;Benhatchi, Karim
目的：莱姆病（LD）是由蜱传播的慢性、多系统人畜共患疾病，LD的病原体是伯氏疏螺旋体复合体的螺旋体。横断面研究的目的是根据斯洛伐克东部患者血清中是否存在特异性抗体来分析 LD 发生率，并比较血清学 ELISA 和免疫印迹测定的结果。 方法：通过对斯洛伐克东部受访者进行现场采样获得带有问卷的静脉血。总体而言，我们通过 ELISA 检查了 537 份人类血清，为了确认，我们测试了针对疏螺旋体免疫印迹测定的所有阳性 IgG 抗体。结果：我们的结果证实了抗疏螺旋体抗体的高血清流行率（IgG 为 17.9%），而 ELISA IgG 阳性血清中 69.8% 的应答者证实了免疫印迹血清阳性测试。 7.6% 的研究人群中发现了 IgM 类阳性抗体。最常见的是针对 VlsE (80.2%)、p41 (66.7%)、p18 (56.3%)、p100 (41.7%)、p58 (31.3%) 和 p39 (30.2%) 的抗体。这只是该细菌在人类 LD 临床症状发展过程中存在的间接证据。实验室LD检测应按照有效标准进行，阳性和不确定的结果必须通过Western Blot/Immunoblot检测来确认。
Objective: Lyme disease (LD) is chronic, multi-system zoonosis transmitted by ticks, and LD aetiological agents are spirochetes of the Borrelia burgdorferi sensu lato complex. The aim of the cross-sectional study was to analyze the LD incidence on the basis of the presence of specific antibodies in the serum of patients in Eastern Slovakia, and to compare the results of serological ELISA and immunoblot assays.Methods: Venous blood with questionnaires was obtained by field sampling of respondents from Eastern Slovakia. Overall, we examined 537 human sera by the ELISA and for confirmation we tested all positive IgG antibodies against the Borrelia immunoblot assay.Results: Our results confirmed the high serum prevalence of anti-Borrelia antibodies (17.9% for IgG), while the immunoblot seropositive test was confirmed in 69.8% of responders from ELISA IgG positive sera. Positive antibodies of the IgM class were found in 7.6% of the population under study. Most commonly found were antibodies against VlsE (80.2%), p41 (66.7%), p18 (56.3%), p100 (41.7%), p58 (31.3%), and p39 (30.2%).Conclusion: It should be noted that detection of antibodies against B. burgdorferi s.l. is only an indirect evidence of the presence of this bacterium in the development of clinical signs of LD in humans. Laboratory LD tests should be performed in accordance with valid standards, positive and uncertain results must be confirmed by the Western Blot/Immunoblot assay.