Rab27A regulates exosome secretion from lung adenocarcinoma cells A549: involvement of EPI64
Rab27A regulates exosome secretion from lung adenocarcinoma cells A549: involvement of EPI64
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DOI：
10.1111/apm.12261
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发表时间：
2014-01-01
期刊：
影响因子：
2.8
通讯作者：
Li, Xiaofei
中科院分区：
文献类型：
--
作者：
Li, Wenhai;Hu, Yunsheng;Li, Xiaofei
外泌体是通过胞吐作用分泌到细胞外区室中的小膜囊泡。外泌体的独特组成可以被转运到其他细胞，使细胞在远处发挥生物学功能。然而，在肺癌中，外泌体分泌的调节知之甚少。本研究以人肺腺癌A549细胞为研究对象，探讨了人肺腺癌A549细胞外泌体的分泌及其调控机制。我们发现Rab 27 A在A549细胞中有表达，Rab 27 A特异性shRNA对Rab 27 A的抑制作用可显著降低A549细胞外泌体的分泌。在A549细胞中也检测到对Rab 27具有特异性的候选GAP EPI 64。通过pull-down实验，我们发现EPI 64是作为Rab 27 A的特异性GAP而不是Rab 27 B参与A549细胞外泌体的分泌。EPI 64的过表达增强了外泌体的分泌。总之，在A549细胞中，EPI 64可以通过作为Rab 27 A特异性的GAP来调节外泌体分泌。
Exosomes are small membrane vesicles secreted into the extracellular compartment by exocytosis. The unique composition of exosomes can be transported to other cells which allow cells to exert biological functions at distant sites. However, in lung cancer, the regulation of exosome secretion was poorly understood. In this study, we employed human lung adenocarcinoma A549 cells to determine the exosome secretion and involved regulation mechanism. We found that Rab27A was expressed in A549 cells and the reduction of Rab27A by Rab27A-specific shRNA could significantly decrease the secretion of exosome by A549 cells. EPI64, a candidate GAP that is specific for Rab27, was also detected in A549 cells. By pull-down assay, we found that EPI64 participated in the exosome secretion of A549 cells by acting as a specific GAP for Rab27A, not Rab27B. Overexpression of EPI64 enhanced exosome secretion. Taken together, in A549 cells, EPI64 could regulate the exosome secretion by functioning as a GAP specific for Rab27A.