High Speed Nonlinear Interferometric Vibrational Analysis of Lipids by Spectral Decomposition
High Speed Nonlinear Interferometric Vibrational Analysis of Lipids by Spectral Decomposition
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DOI：
10.1021/ac100222c
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发表时间：
2010-05-01
影响因子：
7.4
通讯作者：
Gruebele, Martin
中科院分区：
文献类型：
--
作者：
Chowdary, Praveen D.;Benalcazar, Wladimir A.;Gruebele, Martin
与其他基于CARS（相干反斯托克斯拉曼散射）的光谱技术不同，非线性干涉振动光谱（NIVS）在分析物浓度方面是线性的，并且具有没有非共振背景失真的拉曼线形。我们使用自发拉曼散射作为NIVS的高精度基准。作为一个具有挑战性的比较，我们检查光谱中的CH拉伸区域的六个脂质样品。奇异值分解和参考一个独立的化学分析被用来直接比较NIVS和自发拉曼散射。我们证明，NIVS可以确定六种不同的脂质样品的相对不饱和度，准确自发拉曼光谱，但200倍的速度。绘制出皮肤组织样本，以展示具有空间分辨率的定量脂质-蛋白质分化。
Unlike other CARS-based (coherent anti-Stokes Raman scattering) spectroscopy techniques, nonlinear interferometric vibrational spectroscopy (NIVS) is linear in analyte concentration and has a Raman line shape free of non-resonant background distortions. We use spontaneous Raman scattering as a high accuracy benchmark for NIVS. As a challenging comparison, we examine spectra in the CH stretching region of six lipid samples. Singular value decomposition and reference to an independent chemical assay are used to directly compare NIVS and spontaneous Raman scattering. We demonstrate that NIVS can determine the relative degree of unsaturation in six different lipid samples as accurately as spontaneous Raman spectroscopy, but 200 times faster. A skin tissue sample is mapped out to demonstrate quantitative lipid-protein differentiation with spatial resolution.