BOVINE GRANULOSA-CELLS PRODUCE BASIC FIBROBLAST GROWTH-FACTOR
BOVINE GRANULOSA-CELLS PRODUCE BASIC FIBROBLAST GROWTH-FACTOR
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DOI：
10.1210/endo-121-2-597
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发表时间：
1987-08-01
期刊：
影响因子：
4.8
通讯作者：
GOSPODAROWICZ, D
中科院分区：
文献类型：
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作者：
NEUFELD, G;FERRARA, N;GOSPODAROWICZ, D
培养的牛颗粒细胞表达编码碱性成纤维细胞生长因子(BFGF)的基因。碱性成纤维细胞生长因子基因被转录成7.0kb和3.7kb的mRNA转录本，这些转录本显然被翻译成16000molwt的bFGF样生长因子。颗粒细胞来源的碱性成纤维细胞生长因子具有生物活性。可刺激毛细血管内皮细胞或颗粒细胞增殖。这种促有丝分裂的作用可以被特定的中和抗bFGF抗体所阻止。我们的结果表明，颗粒细胞来源的bFGF既可以作为自分泌生长因子，也可以作为旁分泌生长因子，并进一步表明该因子可能参与了卵泡内膜丰富的血管系统的发育。
Cultured bovine granulosa cells express the gene encoding basic fibroblast growth factor (bFGF). The bFGF gene is transcribed into 7.0- and 3.7-kilobase mRNA transcripts which are apparently translated into 16,000 mol wt bFGF-like growth factor. The granulosa cell-derived bFGF is bioactive, ie. it can stimulate the proliferation of capillary endothelial or granulosa cells. This mitogenic effect is prevented by specific neutralizing anti-bFGF antibodies. Our results indicate that bFGF derived from granulosa cells can act as both autocrine and paracrine growth factor, and they further suggest that the factor may be involved in the development of the rich vasculature of the theca interna of the follicle.