Improving docosahexaenoic acid production by Schizochytrium sp using a newly designed high-oxygen-supply bioreactor
Improving docosahexaenoic acid production by Schizochytrium sp using a newly designed high-oxygen-supply bioreactor
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使用新设计的高供氧生物反应器提高裂殖壶菌的二十二碳六烯酸产量
DOI：
10.1002/aic.15783
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发表时间：
2017-10-01
期刊：
影响因子：
3.7
通讯作者：
Huang, He
中科院分区：
文献类型：
--
作者：
Guo, Dong-Sheng;Ji, Xiao-Jun;Huang, He
足够高的氧气供应对于包括裂殖酵母在内的好氧微生物的高细胞密度培养至关重要。为此，我们设计了一种新型的高供氧生物反应器，并对其相关工艺参数和发酵阶段特性进行了研究。通过实时监测发酵过程中pH值和非油脂生物量的变化，作为氮素消耗和脂肪积累状况的指标，首次将其应用于三阶段分批发酵过程。实验结果表明，该多孔膜-叶轮式生物反应器的生物量高于常规生物反应器，而二十二碳六烯酸(DHA)占总脂的百分比低于常规生物反应器。在此基础上，对多孔膜-叶轮式生物反应器进行了分级控制，最大生物量为151.0 g/L，二氢呋喃浓度为44.3g/L，占总生物量的比例为29.33%，产率为369.08 mg/(1h)。本研究为裂殖酵母生产DHA提供了一个高效、经济的生物反应器。(C)2017美国化学工程师学会学报，63：4278-4286,2017
A sufficiently high oxygen supply is crucial for high-cell-density cultivation of aerobic microorganisms, including Schizochytrium sp. We, therefore, designed a novel bioreactor enabling high-level oxygen supply, and its relevant process parameters and fermentation-stage characteristics were investigated. The real-time changes of pH and nonoil biomass were monitored as proxies for the consumption of nitrogen and lipid accumulation status, which was first applied to divided fermentation process with three stages. The experimental results showed that the biomass in this porous-membrane-impeller bioreactor was higher than in conventional bioreactor, while docosahexaenoic acid (DHA) percentage in total lipids was lower than in conventional bioreactor. A multistage control strategy is subsequently implemented for the porous-membrane-impeller bioreactor, and the maximum biomass, DHA concentration, DHA percentage in biomass and DHA productivity reached 151.0 g/L, 44.3 g/L, 29.33%, 369.08 mg/(Lh), respectively. This study thus provides a highly efficient and economic bioreactor for the production of DHA by Schizochytrium sp. (c) 2017 American Institute of Chemical Engineers AIChE J, 63: 4278-4286, 2017