Detection of Staphylococcus aureus enterotoxin B at femtomolar levels with a miniature integrated two-channel surface plasmon resonance (SPR) sensor
Detection of Staphylococcus aureus enterotoxin B at femtomolar levels with a miniature integrated two-channel surface plasmon resonance (SPR) sensor
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DOI：
10.1016/s0956-5663(02)00014-3
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发表时间：
2002-06-01
影响因子：
12.6
通讯作者：
Furlong, CE
中科院分区：
文献类型：
--
作者：
Naimushin, AN;Soelberg, SD;Furlong, CE
表面等离子体共振(SPR)生物传感器提供了连续实时监测的能力。现有的商业仪器体积大、价格昂贵，不适合现场应用。我们在此报告了一种基于双通道原型系统的SPR传感器系统，该系统类似于单通道Spreeta(TM)设备。该系统是现场应用的理想选择。双通道设计提供了一个参考通道来补偿体折射率(RI)、非特定结合和温度变化。SPR软件包括校准功能，可归一化来自两个通道的响应，从而实现准确的参考。此外，利用基于帕尔蒂埃效应的热电模块的温控外壳提供了准确测量RI所需的温度稳定性。完整的SPR传感器系统可以由12V电池供电。预功能化、一次性、镀金的薄玻璃片为系统提供了易于更新的传感器元件。金黄色葡萄球菌肠毒素B(SEB)是一种小蛋白毒素，可在亚纳米分子水平上直接检测到，并可在毫微分子水平上扩增。传感器表面的再生程序允许在1个月内进行60多个直接检测循环。(C)2002 Elsevier Science B.V.保留所有权利。
Surface plasmon resonance (SPR) biosensors offer the capability for continuous real-time monitoring. The commercial instruments available have been large in size, expensive, and not amenable to field applications. We report here an SPR sensor system based on a prototype two-channel system similar to the single channel Spreeta(TM) devices. This system is an ideal candidate for field use. The two-channel design provides a reference channel to compensate for bulk refractive index (RI), non-specific binding and temperature variations. The SPR software includes a calibration function that normalizes the response from both channels, thus enabling accurate referencing. In addition, a temperature-controlled enclosure utilizing a thermo-electric module based on the Peltier effect provides the temperature stability necessary for accurate measurements of RI. The complete SPR sensor system can be powered by a 12V battery. Pre-functionalized, disposable, gold-coated thin glass slides provide easily renewable sensor elements for the system. Staphylococcus aureus enterotoxin B (SEB), a small protein toxin was directly detectable at sub-nanomolar levels and with amplification at femtomolar levels. A regeneration procedure for the sensor surface allowed for over 60 direct detection cycles in a 1-month period. (C) 2002 Elsevier Science B.V. All rights reserved.