Fermentable dietary fiber potentiates the localization of immune cells in the rat large intestinal crypts
Fermentable dietary fiber potentiates the localization of immune cells in the rat large intestinal crypts
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DOI：
10.1177/153537020422900903
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发表时间：
2004-10-01
影响因子：
3.2
通讯作者：
Hara, H
中科院分区：
文献类型：
--
作者：
Ishizuka, S;Tanaka, S;Hara, H
肠隐窝由明确的上皮细胞层次结构组成，增殖的上皮细胞位于靠近隐窝底部的位置，甚至在大肠中也是如此。我们研究了CD8(+)和CD4(+)上皮内淋巴细胞(IEL)和CD161(+)自然杀伤(NK)细胞是否定位于盲肠和结肠的增殖或分化上皮区域。对增殖的上皮层细胞和沿大肠纵隐窝轴的免疫细胞进行组织化学测量。饮食干预表明，免疫细胞在纵隐窝轴中的生理定位取决于免疫细胞类型。 CD8(+) IEL优先位于分化的上皮细胞中。相反，CD161(+) NK 细胞位于隐窝底部的上皮细胞附近。盲肠隐窝含有的 CD8(+) IEL 数量明显多于结肠隐窝。然而，盲肠和结肠上皮细胞中仅存在少量 CD4(+) IEL。一些膳食纤维增加了盲肠中 CD8(+) IEL 和 CD161(+) NK 细胞的密度，不同纤维类型的反应程度不同。 SCFA与免疫细胞的定位存在显着关系，尤其是CD8(+) IEL和CD161(+) NK细胞，这些细胞被认为参与上皮稳态的维持。
Intestinal crypts are composed of a well-defined hierarchy of epithelial cells, and proliferating epithelial cells reside close to the bottom of the crypts-even in the large intestine. We investigated whether CD8(+) and CD4(+) intraepithelial lymphocytes (IELs) and CD161(+) natural killer (NK) cells localized in proliferating or differentiated epithelial region of cecum and colon. Both proliferating epithelial layer cells and the immune cells along the longitudinal crypt axis of the large intestine were measured histochemically. Dietary intervention revealed that the physiological localization of the immune cells in the longitudinal crypt axis depended on the immune cell type. CD8(+) IELs were preferentially located among differentiated epithelial cells. In contrast, CD161(+) NK cells were located adjacent to the epithelial cells at the bottom of crypt. Cecal crypts contained significantly larger numbers of CD8(+) IELs than did colonic crypts. However, there was only a minor population of CD4(+) IEL in the cecal and colonic epithelia. Some dietary fibers increased the densities of CD8(+) IELs and CD161(+) NK cells in the cecum, with the magnitude of response varying among the types of fiber. There was a significant relationship between SCFA and the localization of immune cells, especially CD8(+) IEL and CD161(+) NK cells, which are considered to be involved in the maintenance of epithelial homeostasis.