MONOCLONAL-ANTIBODIES TO BOVINE CORONAVIRUS - CHARACTERISTICS AND TOPOGRAPHICAL MAPPING OF NEUTRALIZING EPITOPES ON THE E2-GLYCOPROTEINS AND E3-GLYCOPROTEINS
MONOCLONAL-ANTIBODIES TO BOVINE CORONAVIRUS - CHARACTERISTICS AND TOPOGRAPHICAL MAPPING OF NEUTRALIZING EPITOPES ON THE E2-GLYCOPROTEINS AND E3-GLYCOPROTEINS
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DOI：
10.1016/0042-6822(87)90134-6
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发表时间：
1987-12-01
期刊：
影响因子：
3.7
通讯作者：
BABIUK, LA
中科院分区：
文献类型：
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作者：
DEREGT, D;BABIUK, LA
制备了牛冠状病毒魁北克分离株的单抗，并对其进行了鉴定。抗E2和E3糖蛋白的单抗在体外被发现能有效地中和病毒。针对E1糖蛋白的单抗均不能中和病毒的感染性。抗E2糖蛋白的中和性单抗均能免疫沉淀gp190、gp100及其胞内前体蛋白gp170。抗E3糖蛋白的中和性单抗免疫沉淀gp124，并与其前体蛋白gp59和gp118显示不同的反应性。这些单抗对E3的一种明显降解产物也表现出不同的反应性。根据竞争结合分析，E2的中和性单抗结合到两个不同的不重叠的抗原域。抗E3糖蛋白的中和性单抗也结合到两个不同的抗原部位，如竞争性结合试验所确定的那样，加上第三个重叠这些区域的部位。其他结果表明，E3糖蛋白上的一个结构域可以进一步细分为两个表位。因此，E3特异性单抗可以确定四个表位。
Monoclonal antibodies to the Quebec isolate of bovine coronavirus were produced and characterized. Monoclonal antibodies to both the E2 and the E3 glycoproteins were found to efficiently neutralize virus in vitro. None of the monoclonal antibodies directed against the E1 glycoprotein neutralized virus infectivity. Neutralizing monoclonal antibodies to the E2 glycoprotein were all found to immunoprecipitate gp190, gp100, and their intracellular precursor protein gp170. Neutralizing monoclonal antibodies to the E3 glycoprotein immunoprecipitated gp124 and showed differential reactivity to its precursor proteins gp59 and gp118. These monoclonal antibodies also showed differential reactivity to an apparent degradation product of E3. Neutralizing monoclonal antibodies to E2 bound to two distinct nonoverlapping antigenic domains as defined by competitive binding assays. Neutralizing monoclonal antibodies to the E3 glycoprotein also bound to two distinct antigenic sites as defined by competitive binding assays plus a third site which overlapped these regions. Other results indicated that the one domain on the E3 glycoprotein could be further subdivided into two epitopes. Thus four epitopes could be defined by E3-specific monoclonal antibodies.