Gene expression analysis of host innate immune responses in the central nervous system following lethal CVS-11 infection in mice.
Gene expression analysis of host innate immune responses in the central nervous system following lethal CVS-11 infection in mice.
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DOI：
10.7883/yoken.64.463
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发表时间：
2011-11
影响因子：
2.2
通讯作者：
Naoko Sugiura;A. Uda;S. Inoue;D. Kojima;N. Hamamoto;Y. Kaku;A. Okutani;Akira Noguchi;Chun-Ho Park;A. Yamada
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文献类型：
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作者：
Naoko Sugiura;A. Uda;S. Inoue;D. Kojima;N. Hamamoto;Y. Kaku;A. Okutani;Akira Noguchi;Chun-Ho Park;A. Yamada
应用基因芯片和经典途径分析技术，对狂犬病病毒CVS-11株（RABV）感染小鼠的中枢神经系统（CNS）组织进行基因表达分析。与先天免疫以及炎症反应相关的基因显著上调，这与先前使用减毒病毒获得的发现相印证，减毒病毒不会在感染小鼠中诱导致命结果。组织学检查显示小脑神经元发生凋亡。虽然Fas配体上调的程度不是那么突出，穿孔素和颗粒酶基因在CVS-11感染的小鼠的CNS中高度表达。浦肯野细胞和CD 3 T淋巴细胞中穿孔素和颗粒酶的存在强烈表明前者细胞的凋亡是由后者细胞诱导的。
The central nervous system (CNS) tissue of mice infected with the CVS-11 strain of rabies virus (RABV) was subjected to gene expression analysis using microarray and canonical pathway analyses. Genes associated with innate immunity as well as inflammatory responses were significantly up-regulated, corroborating with the previous findings obtained using attenuated viruses that did not induce a fatal outcome in infected mice. Histopathological examination showed that neurons in the cerebellum had undergone apoptosis. Although the extent of Fas ligand up-regulation was not so prominent, perforin and granzyme genes were highly expressed in the CNS of mice infected with CVS-11. The presence of perforin and granzymes both in the Purkinje cells and CD3 T lymphocytes strongly suggested that apoptosis of the former cells was induced by the latter cells.