INTRACELLULAR-RECORDING OF IDENTIFIED NEOSTRIATAL PATCH AND MATRIX SPINY CELLS IN A SLICE PREPARATION PRESERVING CORTICAL INPUTS
INTRACELLULAR-RECORDING OF IDENTIFIED NEOSTRIATAL PATCH AND MATRIX SPINY CELLS IN A SLICE PREPARATION PRESERVING CORTICAL INPUTS
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DOI：
10.1152/jn.1989.62.5.1052
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发表时间：
1989-11-01
影响因子：
2.5
通讯作者：
EMSON, PC
中科院分区：
文献类型：
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作者：
KAWAGUCHI, Y;WILSON, CJ;EMSON, PC
1. 采用保留内侧颗粒皮层输入的体外切片制备方法，比较了大鼠两组新纹状体投射细胞、斑块细胞和基质棘细胞的形态学、电膜特性和皮质纹状体兴奋性突触后电位（EPSPs）。用生物细胞素对刺细胞进行细胞内染色，并通过免疫组化对同一切片上28kd钙结合蛋白calbindin进行鉴定，确定刺细胞属于斑块（纹状体）室或基质。2. 斑块神经元和基质神经元具有非常相似的轴突和树突形态。斑块细胞和基质细胞的树突和局部轴突侧枝几乎都在各自的室室中伸展。斑块细胞和大多数基质细胞在母体树突区域内或附近有局部轴突侧枝。然而，有一类基质细胞将轴突侧枝延伸到新纹状体的更宽部分，但仍局限于基质室。3. 根据膜对细胞内施加电流脉冲的响应估计输入电阻和膜时间常数。基质细胞的平均值为8.41 ms。膜片细胞的值为31.8 M ω和8.19 ms，与基质细胞的值一致。当使用电流脉冲进行测试时，两种类型的细胞都显示出相同的膜非线性。输入电阻和时间常数都受到快速异常整流的强烈影响，因此与电压相关，随着膜极化而减小。在去极化电流阶跃下观察到缓慢的斜坡状去极化响应。4. 使用去极化电流脉冲检查了重复放电。在两种类型的棘细胞中，动作电位序列对尖峰频率的适应不大，当电流强度小于1 nA时，动作电位序列呈线性增加。从第一次和第二次间隔计算得到的斜率频率，基质细胞分别为115.0和107.2 Hz/nA，斑块细胞为86.0和82.8 Hz/nA。5. 内侧颗粒皮层的刺激在两个区室的一些纹状体细胞中诱导EPSPs。基质细胞中的EPSP通常同时表现出短潜伏期和长潜伏期，这与体内观察到的两种早期反应相对应。部分基质细胞和所有斑块细胞仅表现出较长潜伏期的EPSP成分。基质细胞的平均潜伏期为6.3 ms，斑块细胞的平均潜伏期为9.1 ms。EPSP振幅与膜电位呈非线性关系，EPSP振幅和持续时间随膜极化的减小而增大。（摘要删节为400字）
1. The morphology, electrical membrane properties, and corticostriatal excitatory postsynaptic potentials (EPSPs) of two groups of neostriatal projection cells, patch cells, and matrix spiny cells were compared in the rat by the use of an in vitro slice preparation that preserves inputs from medial agranular cortex. Spiny cells were stained intracellularly with biocytin and identified as belonging to the patch (striosomal) compartment or to the matrix by immunohistochemistry for the 28 kD calcium-binding protein calbindin on the same sections. 2. Patch and matrix neurons had very similar axonal and dendritic morphology. Both patch and matrix cells extended their dendrites and local axon collaterals almost exclusively in their respective compartments. Patch cells and most matrix cells had local axon collaterals within or near the parent dendritic domain. However there was a class of matrix cells that extended axon collaterals over a much wider portion of the neostriatum but still restricted to the matrix compartment. 3. Input resistance and membrane time constant were estimated from the membrane response to intracellularly applied current pulses. The average values of matrix cells were and 8.41 ms. The values of patch cells were 31.8 M omega and 8.19 ms and were within the range of those of matrix cells. Both types of cells showed the same kinds of membrane nonlinearities when tested with the use of current pulses. Input resistance and time constant were both strongly affected by a fast anomalous rectification and were thus voltage-dependent, decreasing with membrane polarization. Slow ramplike depolarizing responses were observed in response to depolarizing current steps. 4. Repetitive firing was examined with the use of depolarizing current pulses. In both types of spiny cells, trains of action potentials showed little adaptation of spike frequency and linearly increased with current intensities less than 1 nA. The slopes frequency, calculated from the first and second intervals, were 115.0 and 107.2 Hz/nA, respectively, for matrix cells and 86.0 and 82.8 Hz/nA for patch cells. 5. Stimulation of the medial agranular cortex induced EPSPs in some striatal cells in both compartments. EPSP in matrix cells often showed both short-latency and long-latency components, corresponding to two early components of the response observed in vivo. Some matrix cells, and all patch cells, showed only the longer latency EPSP component. The average latency was 6.3 ms in matrix cells and 9.1 ms in patch cells. The relationship between EPSP amplitude and membrane potential was nonlinear, with EPSP amplitude and duration increasing with decreasing membrane polarization.(ABSTRACT TRUNCATED AT 400 WORDS)